Covaris LE220 User Manual

Covaris
LE220 (-plus, R-plus, and Rsc) Setup & Instruction Manual
Part Number: 010398 Rev H | November 2019
LE220 (-plus, R-plus, and Rsc)
Setup & Instruction Manual
Instrument and Software Developed for High-performance Sample
Preparation using Adaptive Focused Acoustic
®
(AFA
®
) Energy
For Research Only
Model Numbers: LE220-plus, LE220R-plus, and LE220Rsc | Software: SonoLab 8

Covaris LE220 Specifications

General

Type	Ultrasonic Liquid Processor
Frequency	500 kHz
Voltage	100-240 VAC
Applications	DNA shearing
Sample Types	Biological samples

Summary

UNIVERSAL PRECAUTIONS

Warnings

Warranty

2. Installation and Setup

Assembly

Steps for unpacking and assembling the instrument and accessories.

3. Getting Started

Fill the Water Bath

Instructions for filling the instrument's water bath.

Start the System

Steps to power on and initialize the instrument and software.

System Preparation

Running a Method

Daily System Shutdown

4. Create and Edit Methods

Create New Method

Guide to creating new experimental methods using the editor.

Add Method Step

Choosing a Treatment Step

Defining acoustic parameters for a treatment step.

Define Treatment Parameters

Create Methods Using Repeat Function

Scanning Treatments (LE220Rsc Only)

Create Scanning Treatment

Modify Existing Methods

5. SonoLab Application Software

6. System Specifications

7. Maintenance

Transducer Handling

Proper procedures for handling and cleaning the transducer.

System Checks and Cleaning

System Shutdown Procedures

Steps for long-term storage and powering down the system.

8. Troubleshooting

Instrument Connectivity Issues

Steps to resolve problems with SonoLab connection.

Safety and Sensor Failures

Diagnosing issues with interlocks and sensors.

Analysis Result Problems

System Errors and Support

Addendum for LE220Rsc