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diagenode Bioruptor Pico User Manual

diagenode Bioruptor Pico
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Bioruptor
®
Pico
Sonication System
Version 3 01_2020
USER GUIDE

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diagenode Bioruptor Pico Specifications

General IconGeneral
TypeUltrasonic Homogenizer
Voltage100-240 V
ApplicationDNA Shearing
Shearing TimeProgrammable (1s to 99 min)
Sonication TimeProgrammable (1s to 99 min)

Summary

Guarantee and Support Information

Limited 5 years global warranty

Details the warranty terms, duration, and coverage for the Bioruptor Pico.

Critical steps for maintenance and efficient shearing

General warnings and best practices

Essential warnings and recommended practices for safe and efficient operation.

Sonication bath temperature management

Optimal temperature range for the sonication bath and the role of the Bioruptor Cooler.

Validated tubes and fitting instructions

Lists compatible tubes and provides instructions for fitting them into the tube holder.

Getting to know your Bioruptor Pico system

Bioruptor Pico components overview

Visual overview and identification of the main components of the Bioruptor Pico.

Sonication bath operation

Handling and water quality

Guidelines for handling the unit and maintaining water quality in the sonication bath.

Water temperature control

Importance of maintaining 4°C for optimal sonication and the role of automatic temperature control.

Bioruptor Pico accessories

Tube holders and sample volumes

Details available tube holders and their minimum/maximum sample volume capacities.

Equipment installation

Safe operating conditions

Lists the environmental and electrical conditions required for safe device operation.

Installation overview diagram

Schematic diagram illustrating the installation of the Bioruptor Pico with its cooler.

Installing the Bioruptor Pico system

System operation

Touch screen interface overview

Overview of the touch screen interface for programming sonication protocols, including main functions.

Bioruptor Pico interface features

Main interface functions

Details core interface options like Go & Shear, User Protocols, Guidelines, and Good Practices.

Starting a shearing experiment

Entering experiment parameters

Inputting sonication ON/OFF times, number of cycles, and tube type or frequency.

Preparing samples for sonication

Processing and monitoring sonication

Initiating and monitoring the run

Starting the shearing process, understanding the running screen, and progress monitoring.

Tube holders and consumables

Safety information

General safety guidelines

Essential safety instructions for intended use, non-intended use, and foreseeable misuse.

Device handling and environment

Personnel and safety instruction definitions

Circulation chiller design - Front side

Circulation chiller design - Back side

Chiller controls

Chiller function indicators

Device placement requirements

Critical stability warning

Important warning regarding device stability to prevent rolling away or falling over.

Commissioning procedures

Device startup and filling

Critical safety warnings during filling

Highlights dangers of compressor overheating, incorrect liquid, overflow, and spraying.

Heat transfer liquid level management

Device operation modes

Maintenance procedures

General maintenance safety

Crucial safety precautions for contact with live parts, hot/cold components, and liquids.

Device cleaning and component replacement

Troubleshooting faults and errors

Decommissioning and draining

Disposal instructions

Caution: Refrigerant handling

Cautionary advice regarding uncontrolled refrigerant escape and specialist decommissioning.