This document describes the HOLTEN SAFE 2000 and MAXI SAFE 2000 Class II biological safety cabinets, designed to protect the operator, surroundings, and work process from particle/microbiological contamination. These cabinets comply with various international standards, including NSF Std 49, Nordiska R3-Föreningens Norm for säkerhetsbänkar, DIN 12950 Teil 10, NF 44/201, and BS 5726 (92).
Function Description
The HOLTEN SAFE 2000 and MAXI SAFE 2000 cabinets are microprocessor-controlled Class II biological safety cabinets. They provide protection by maintaining a controlled airflow that prevents the escape of hazardous particles and microorganisms from the work chamber, while also protecting the product from external contamination. The design incorporates a negative pressure plenum for enhanced operator and product safety.
Key components and their functions:
- Exhaust filter (1): Filters air exiting the cabinet.
- Chanal hose PA (2): Likely refers to a channel or duct for air.
- Fan (3): Drives the airflow within the cabinet.
- Main filter (4): Filters air for the work chamber.
- Control panel (5): Provides user interface for operation and settings.
- Fluorescent light with reflector (6): Illuminates the work chamber.
- Push up front opening (7): Refers to the motor-driven front window.
- Side window (8): Provides light and visibility.
- Stainless trough (maxisafe with drain) (9): Work surface with a drain for liquids.
- Prefilter (only maxisafe) (10): Additional filter for MAXI SAFE models.
- Support stand (11): Base for the cabinet.
Important Technical Specifications
The HOLTEN SAFE 2000/MAXI SAFE 2000 series comes in several models (0.9, 1.2, 1.5, 1.8), with varying dimensions and capacities.
General Specifications (for all models unless specified):
- HEPA filter efficiency: 99.999%
- Air velocity downflow: 0.4 m/sec
- Voltage/frequency: 230 V / 50 Hz
- Light intensity: 1400 Lux
- Sound level max.: 60 dBA
- Noise level (ISO): 58-59 dBA
- Power: 2 A (models 0.9, 1.2), 3.5 A (models 1.5, 1.8)
- Power consumption: 0.5 kW (models 0.9, 1.2), 0.8 kW (models 1.5, 1.8)
Model-Specific Dimensions and Weights:
| Model |
External Dimensions (DxWxH) |
Chamber Dimensions (DxWxH) |
Weight (Safe) |
Weight (Maxi Safe) |
Exhaust Air Volume |
Spillage (Safe) |
Spillage (Maxi Safe) |
| 0.9 |
867x1020x2000 mm |
580x900x700 mm |
190 kg |
215 kg |
300 m³/h |
13.0 l |
4.5 l |
| 1.2 |
867x1320x2000 mm |
580x1200x700 mm |
200 kg |
225 kg |
400 m³/h |
18.0 l |
6.0 l |
| 1.5 |
867x1620x2000 mm |
580x1500x700 mm |
215 kg |
290 kg |
500 m³/h |
22.0 l |
7.5 l |
| 1.8 |
867x1920x2000 mm |
580x1800x700 mm |
250 kg |
300 kg |
600 m³/h |
27.0 l |
9.0 l |
Materials:
- Front windows and side windows: Glass (laminated/hardened)
- Window frames: Stainless steel AISI 304 (polished)
- Upper part, return duct and bottom part: Steel plate 1203 (polyester-coated)
- Stand: Steel plate 1203 (polyester-coated)
- Trough, table tops and suction gate: Stainless steel AISI 304 (polished)
Usage Features
The cabinet is designed for ease of use and safety, featuring a microprocessor controller with a clear LCD display.
Control Panel (Fig. A):
- Button ON/OFF for UV light (1): Activates UV light (optional), only when chamber light is off and front window is in the lowest position.
- Button ON/OFF for chamber light (2): Controls the internal light.
- [MENU] button (3): Used to enter UV, FAN, or TIME mode. A flashing icon indicates the selected mode.
- [PLUS] button (4): Sets minutes, hours, and day of the week.
- Button for hoisting the front window up (5): Raises the motor-driven front window.
- Button to stop acoustic alarm (6): Silences alarms.
- Button to activate fan (7):
- Once: Normal fan speed.
- Twice: Reduced fan speed.
- Three times: Fan off.
- [ENTER] button (8): Confirms selections during settings.
- Key switch (9): Activates/deactivates start/stop button and switches between reduced or normal fan speed.
- Button to lower the front window (10): Lowers the motor-driven front window.
LCD Display (Fig. B):
- Speed of fan (11): Indicates current fan speed.
- Front window (12): Shows front window status.
- Indication regarding filter change (13): Alerts when filter change is needed.
- Selected days with automatic start up (14): Shows programmed auto-start days.
- FAN alarm (15): Indicates fan malfunction.
- UV-timer active (16): Shows when UV timer is running.
- FAN timer active (17): Shows when FAN timer is running.
- Stop watch mode (18): Indicates stopwatch mode.
- UV icon (19): Appears when UV mode is selected.
- FAN icon (20): Appears when FAN mode is selected.
- Stop watch active (21): Shows when stopwatch is running.
Ergonomics:
- Ergonomically correct sloping front for comfortable work, standing or sitting.
- Side windows for perfect light conditions and view.
- Motor-driven double function front window with both sliding and hinged modes.
Advanced Features:
- Clock: For pre-setting automatic start-up, UV-timer, and hour counter.
- Alarm for malfunction: Acoustic and visual alarms for critical conditions.
- "AOS" (Automatic Operation System): Optional accessory for automatic fan start/stop when the sensor is activated by the operator.
- Adjustable FAN speeds: Allows selection of FAN off, 1/2, or 1/1 speed.
- Key switch: For pre-selection of desired fan speeds eligible for the user.
Setting Time and Day of the Week:
- Turn lock-switch to position 1.
- Press [ENTER] while connecting power.
- Press [MENU] to flash minutes, use [+] to set.
- Press [MENU] to flash hours, use [+] to set.
- Press [MENU] to flash days, use [+] to set.
- Press [ENTER] to return to normal mode.
Setting UV Timer:
- Place front window in closed position.
- Turn UV light on by pressing [UV] button.
- Press [MENU] to reach UV position (UV icon appears).
- Press [ENTER] to flash minutes, use [+] to set (1-59 minutes).
- Press [MENU] to flash hours, use [+] to set (1-23 hours).
- Press [ENTER] to store values.
- Press [UV] to start countdown.
Setting FAN Timer:
- Press [MENU] to reach FAN position (FAN icon appears).
- Follow steps 2-5 of UV timer for setting minutes and hours.
- Press [ENTER] to store values and select weekdays for automatic start-up using [+].
- Press [ENTER] to store selected days.
- Press [MENU] to store chosen values (FAN timer icon appears).
Setting Stopwatch:
- Press [MENU] to reach stopwatch position (stopwatch icon appears).
- Follow steps 2-5 of UV timer for setting minutes and hours.
- Press [ENTER] to start countdown. Acoustic alarm sounds at 00:00.
Alarm Conditions:
The acoustic alarm sounds and display light flashes when:
- Fan is running at reduced speed.
- Front window is not in working position.
- Hinged window is not completely closed or in incorrect position.
- Stopwatch counts down to 00:00.
Press button (6) to silence the alarm.
Lock Switch Functionality:
The key switch (9) allows authorized personnel to pre-select eligible fan speeds for users (position 0).
- Position 1: User can select normal, reduced, or off fan speed.
- At priority (with key switch in position 1):
- Press [FAN] once: Fan runs only at normal speed.
- Press [FAN] twice: Operator can choose between normal and reduced speeds.
- Press [FAN] three times: Locks fan in stop mode.
- After adjustment, turn back the lock switch and remove the key.
Power Failure:
If 1/1 FAN speed is selected with the key switch, the cabinet will always restart at full fan speed after a power failure, and the internal light will be switched off.
Maintenance Features
Regular maintenance is crucial for reliable operation and compliance with safety standards.
Recommended Maintenance:
- DAILY: Disinfect the work area, lift tabletops, and wipe the backside and trough.
- WEEKLY: Wipe the exterior of the unit with a mild household detergent.
- REGULARLY (by qualified technician):
- Test correct air velocities.
- Test efficiency of installed HEPA filters.
- Test tightness of the construction.
- These tests should be performed at intervals of approximately 5000 hours of operation or at least once a year.
Filter Change Indication:
The LCD display provides a clear indication for filter change (Fig. 4). A flow graph illuminates in all segments when the filter is new and clean. As the filter becomes contaminated, segments will turn off, indicating the need for replacement.
Cleaning the Inside Front Window:
- Bring the front window to the highest position using button (5).
- Open the front shield.
- Clean the inside window.
Safety Precautions for Installation:
- Ensure the installation site is draught-free and away from frequent pedestrian traffic.
- Mount stainless steel tabletops over the trough.
- Adjust levelling screws for a horizontal and level tabletop.
- Connect the cabinet to the Mains, ensuring compliance with supply specifications and protection by a high-sensitive leakage current contact breaker.
- Valves for gases, vacuum, or liquids are positioned in a separate plate in the side window and should be connected by an authorized technician.
Important Notes for Operation:
- The cabinet should be used indoors in a normal clinical environment (18-28 °C, normal humidity, atmospheric pressure).
- Not for outdoor use or environments with high humidity/pollution.
- Not constructed to ventilate vapours with acid or organic solvents; not a fume hood.
- Switch on fan at normal speed 10 minutes prior to usage.
- Clean and disinfect work chamber and windows with 70% ethanol or similar lint-free wipe.
- Clean/disinfect objects before introducing them into the work chamber.
- Front window must be in working position during work.
- Place product behind front suction holes, as far back as possible.
- Work with steady/soft movements.
- Never overload the work chamber.
- Reduce transfers into and out of the work chamber.
- Avoid products with strong heat emission.
- Do not position the cabinet in places with direct draught or frequent passing of persons.
Biohazard Work:
- Never perform work with the fan at reduced speed.
- The cabinet should not be used for group 4 pathogens or HIGH-RISK biological agents (Class 4 by CDC, oncogenic viruses by NCI).
- Never operate without the fan compartment cover in place to ensure personal protection and prevent contact with rotating fan.
Disinfectants:
- Avoid products containing iodine, chloramine, chlorine dioxide, hypochloride, phenol, and perchloric acid.
- Recommended disinfectants contain alcohol or aldehydes:
- 70% (volume) pure ethanol
- 2% (volume) glutaric aldehyde
- 15% (volume) formaldehyde
- Holten LaminAir does not recommend other disinfectants and is not responsible for their effectiveness or material deterioration.
Need help?
General
To Next Page