Drying thick gels (> 1.5 mm), high concentration
gels, gradient gels on paper
1
Lay a sheet of filter paper on top of the filter paper
liner and then position the gel on this sheet, taking
care to avoid trapping air beneath the gel.
2
Cover the gel with the porous polyethylene cover
sheet, with the smooth side toward the gel.
3
Check that the edges of all the sheets fall within the
recess of the platen.
If necessary, trim the sheets to fit within the recess.
4
Cover the stack with the clear silicone rubber
sealing sheet.
•
p11
Fig 5. Stacks for drying
thick gels to filter paper for
autoradiography.
rubber sealing sheet
stiff plastic cover sheet
porous cellophane
gel
porous cellophane
filter paper liner
stainless steel screen
rubber sealing sheet
stiff plastic cover sheet*
thin plastic wrap
gel thickness ≤ 1.5 mm
filter paper
filter paper liner
stainless steel screen
*not used with agarose gels
rubber sealing sheet
porous polyethylene cover sheet
gel thickness > 1.5 mm
filter paper
filter paper liner
stainless steel screen
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