•
p13
Step 4: Set the timer
The amount of time required for a gel to dry
depends on such factors as gel thickness, gel
concentration, drying temperature, and vacuum
applied. A typical sequencing or 1.5 mm 10%
T gel can be expected to dry in approximately
45 minutes at 80 °C. Larger gels may take 2 to
3 hours. When dry, the thickness of an agarose
gel seen through the silicone flap decreases to
about 1 mm.
1
Press the Time/Temp key to go to the Time mode.
The LED display reads 0:FF and the LED colon blinks
to indicate Elapsed Time.
2
Press either the Up or Down key once to go from
Elapsed Time to Set Time.
3
Press either the Up or Down key to change the
Set Time.
Press Up once to set time for a continuous run. On
a continuous run, the LED display reads r:un and
the heater and vacuum run continuously until you
manually turn them both off.
Press Up again to count up in 15-minute intervals.
Press the Up and Down keys simultaneously to reset
the time to 0. When the Set Time is set to 0, the
display reads 0:FF.
When you have finished setting the time, you
can start the heater and vacuum.
Note: Agarose gels become brittle
when over-dried.
To Next Page
Loading...