6
Complete the stack
a) Place the pre-wet membrane onto the stack of
blotting paper.
b) Place the gel on the membrane. Note: Proteins bind
to the membrane as soon as contact occurs, so it is
important to place the gel correctly on the first try.
c) Cover the gel with three layers of buffer saturated
blotting paper.
•
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Important! Stack each layer with
care, with edges parallel. As each
layer is added, remove all air pock
-
ets by rolling a clean pipet from
the center to the edges. Add a few
drops of buffer to trouble areas to
help remove air pockets.
Mask (opening approx. 2 mm smaller
than the gel on all sides). Optional.
Blotting paper
(2–3 sheets)
Blotting paper
(2– 3 sheets)
Gel
Membrane
These stack compo-
nents are the same
size as the gel or
slightly smaller
Fig 2. Transfer stack for a
single gel.
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