Need help?Do you have a question about the Hoefer TE70X and is the answer not in the manual?
General| Gel Thickness | 0.75 mm or 1.5 mm |
|---|---|
| Type | Vertical Electrophoresis |
| Number of Combs | 1 |
| Number of Wells | 20 wells |
| Current Range | 0.01-1.5 A |
| Power Supply Compatibility | Compatible with most standard power supplies |
Details Hoefer, Inc.'s product warranty terms and limitations of liability.
Guidelines for safe operation, proper accessory use, and preventing damage to the unit.
Rinse electrodes with distilled water before use.
Cut away wells/stacking gel and equilibrate in buffer.
Stack blot paper, membrane, and gel layers carefully.
Cut and saturate blotting paper with transfer buffer.
Cut and pre-wet membrane according to type.
Assemble the transfer stack with gel, membrane, and blotting paper.
Place the cover on the transfer stack and do not remove until complete.
Methods for transferring multiple gels side-by-side or stacked.
Connect leads and set power supply controls to zero.
Calculate current based on gel surface area (0.8 mA/cm²).
Set timer for transfer duration, up to two hours.
Using the keypad to set current, time, and voltage for the transfer.
Monitoring transfer progress using LED, changing parameters, and handling buffer depletion errors.
Detecting and resolving buffer depletion errors ('dry' message).
Turn off power supply and disconnect leads.
Unit beeps; display shows final parameters.
Steps for safely disassembling the transfer stack and removing components.
Instructions on cleaning the unit, avoiding water immersion, and using appropriate cleaning agents.
Solutions for blank or faint areas on the membrane.
Troubleshooting steps for molecules failing to migrate out of the gel.
Solutions for no current output or open circuits in the unit.
Causes and solutions for smeared or diffuse band patterns.
Addressing issues with uneven band transfer.
Troubleshooting chemical and membrane parameters for binding.
Resolving issues with current readings and output.
Addressing 'dry' errors and no output voltage.
Recommendations for optimizing the electrotransfer process.
Methanol concentration guidelines for different membrane types.
Using low ionic strength buffers to prevent overheating.
Recipe and preparation instructions for Towbin buffer.
Recipe and preparation instructions for CAPS buffer.
Factors influencing transfer efficiency and optimization tips.
Information on obtaining technical support, service, and repair for Hoefer products.
Part numbers and order codes for transfer units.
Part numbers and order codes for accessories.
List of related Hoefer electrophoresis systems and power supplies.
Details and order numbers for nitrocellulose and PVDF membranes.
Common reagents for electrophoresis and their order numbers.
To Next Page