um TE70X-IMA0
•
p10
Electrotransfer
TE70X and TE77X
1
The power supply should be switched off and both the
current and voltage controls set at zero
Then plug the color-coded leads from the base of the
transfer unit into the power supply jacks, matching
red to red and black to black. Do not reverse polar-
ity. The lid contains the black, or negative electrode.
The base contains the red, or positve electrode.
2
Set the power supply current
The maximum current setting should not exceed
0.8 mA/cm
2
of the gel surface. If transferring several
layers of gels, a longer transfer time may be required.
Use the graph below to quickly find the current
setting for your gel size, or calculate the gel area
(cm
2
) and multiply it by 0.8 mA/cm
2
.
3
If available, set the power supply timer
Most transfers are complete within one hour, but
larger proteins, proteins from native gels, and thicker
gels may require an additional 1 hour of transfer time.
The optimum transfer time for each protein and gel
system must be determined empirically.
Fig 5. Recommended current
settings for different gel sizes.
Note: Generally, smaller
fragments transfer more
quickly than larger ones.
Note: Transfers exceeding one
hour may require additional
sheets of buffer saturated blot
paper in the transfer stack.
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