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Hoefer TE70X - Post-Transfer Procedures; Disconnecting Power; Unit Beep and Display; Stack Disassembly and Component Removal

Hoefer TE70X
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After the transfer is complete
TE70X and TE77X
Turn off the power supply and disconnect the
leads from the power supply.
TE70XP and TE77XP
After a transfer is complete, the unit will beep
for 5 seconds. The LED display will blink and
cycle between the values for the current, time
and voltage at the time the transfer was stopped.
These can be recorded into a notebook, if desired.
Pushing any button will clear the display.
1
Remove the cover slowly because the stack may
adhere to it.
2
Remove and dispose of the upper blot papers.
3
Remove the gel(s).
Optional: Stain gel to check for residual protein left
in the gel.
4
If desired, label the gel contact side of the membrane
with a soft pencil.
5
Remove the membrane(s) from the stack with
blunt forceps. Process the membrane according to
your protocol or allow the membrane to air dry prior
to storage.
6
Remove the remaining blot papers and dispose.
7
Rinse the unit according to the Care and maintenance
instructions (page 14).
Note: Staining the gel(s) for
residual protein gives an indication
of the completeness of transfer.
Note: Rewet dried membranes in
the appropriate wetting buffer
prior to processing.