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horiba FluoroMax-3 User Manual

horiba FluoroMax-3
220 pages
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FluoroMax-3 v. 3.1 (3 Mar 2006)
i
FluoroMax
®
-3 & FluoroMax
®
-P
with FluorEssence™
Operation Manual
http://www.jobinyvon.com
Rev. 3.1
USA:
HORIBA Jobin Yvon Inc., 3880 Park Avenue, Edison, NJ 08820-3012, Toll-Free:
+1-866-jobinyvon
Tel: +1-732-494-8660, Fax: +1-732-549-5125, E-mail: info@jobinyvon.com, www.jobinyvon.com
France:
HORIBA Jobin Yvon S.A.S., 16-18, rue du Canal, 91165 Longjumeau Cedex,
Tel: +33 (0) 1 64 54 13 00
,
Fax: +33 (0) 1 69 09 93 19, www.jobinyvon.fr
Japan:
HORIBA Ltd., JY Optical Sales Dept, Higashi-Kanda, Daiji Building, 1-7-8 Higashi-Kanda
Chiyoda-ku, Tokyo 101-0031, Tel: +81 (0) 3 3861 8231, www.jyhoriba.jp
Germany:
+49 (0) 89 462317-0
Italy:
+39 0 2 57603050
UK:
+44 (0) 20 8204 8142
China:
+86 (0) 10 6849 2216
(All HORIBA Jobin Yvon companies were formerly known as Jobin Yvon)

Table of Contents

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horiba FluoroMax-3 Specifications

General IconGeneral
Emission Wavelength Range250-900 nm
Excitation SourceXenon Lamp
DetectorPMT (photomultiplier tube)
Sample CompartmentStandard and temperature-controlled options available
Spectral Resolution1.0 nm

Summary

Introduction to the FluoroMax® System

About the FluoroMax®-3 and FluoroMax®-P

Overview of the spectrofluorometer systems and their main components.

Chapter Overview

Provides a summary of the manual's chapters and their content.

Disclaimer and User Responsibilities

Outlines user responsibilities, product limitations, and warranty information.

Requirements and Installation

Safety Training and Environmental Requirements

Details essential safety training and required environmental conditions for operation.

Surface and Electrical Requirements

Specifies necessary surface, clearance, and electrical power configurations.

Unpacking and Initial Setup

Guides through unpacking the system and its components for initial setup.

System Description

Instrument Overview and Operating Theory

Introduces the spectrofluorometer and explains its basic operational principles.

Optical Layout and System Components

Details the physical arrangement and key optical components of the system.

System Detectors and Electronics

Describes the system's detectors and main electronic boards and controllers.

System Operation

Instrument Controls and Power-Up Sequence

Identifies main controls, indicators, and guides the power-on procedure.

System Performance Checks and Calibration

Outlines procedures for verifying system performance and calibration.

Data Acquisition

FluorEssence™ Data Acquisition Tools

Introduces main buttons for data recording and presentation in FluorEssence™.

Spectral Acquisition Procedures

Guides on using the Experiment Menu for spectral scan setup.

Advanced Data Acquisition Tools

Details tools for 3D scan conversion and retrieving peak information.

Acquiring Data for Unknown Samples

Provides a method for finding optimal excitation and emission wavelengths.

Optimizing Data

Cuvette Preparation and Sample Handling

Guides on preparing cuvettes and handling various sample types.

Scan Procedures and Data Optimization Techniques

Covers scan precautions, filters, G factor, S/N, smoothing, and data correction.

Maintenance

Lamp Replacement Procedure

Provides detailed steps and precautions for replacing the xenon lamp.

System Electronics and Component Overview

Describes main electronic components like power supply, CTI card, and controllers.

Troubleshooting

General Troubleshooting Guide and Common Issues

Offers a chart of common problems, causes, and remedies for system issues.

Specific Troubleshooting Scenarios and Solutions

Covers BIOS checks, diagnostic spectra, water Raman analysis, and further assistance.

Producing Correction Factors

Generating and Calculating Correction Factors

Outlines the process for creating emission and excitation correction factors.

Using Correction Factor Files in FluorEssence™

Guides on loading and applying acquired correction factor files.

FluoroMax®-P Phosphorimeter Operation

Phosphorimeter Overview, Theory, and Parameters

Introduces the phosphorimeter, its theory, and key operational parameters.

Phosphorimeter Applications and Data Processing

Describes applications like decay curves and processing phosphorimeter data.

Phosphorimeter Lamp Replacement

Details the procedure for replacing the phosphorimeter's flash lamp.

Automated Polarizers

Polarizer Theory and Measurement Principles

Explains polarization measurement theory, geometries, and magic-angle conditions.

Polarizer Installation and Alignment Procedures

Guides on installing, aligning, and re-aligning the automated polarizers.

Using Automated Polarizers and Analysis

Discusses software usage for polarizer control and polarization analysis.

Polarizer Troubleshooting

Provides solutions for common issues encountered with the automated polarizers.

Technical Specifications

Spectrofluorometer System Specifications

Lists detailed technical specifications for the spectrofluorometer components.

Minimum Computer and Software Requirements

Specifies the necessary computer hardware and software for system operation.

Components & Accessories

Accessory Overview and Itemized List

Provides a list of all available accessories and their corresponding page numbers.

Sample Handling and Measurement Accessories

Details accessories for sample manipulation, temperature control, and measurement.

Light Source, Optical Components, and Upgrades

Describes lamps, filters, fiber optics, and system upgrade options.

Glossary

Glossary of Technical Terms

Defines key terms used throughout the manual for better understanding.

Bibliography

Cited References and Relevant Journals

Lists references and journals related to fluorescence spectroscopy.

Declaration of Conformity

Product Compliance and Standards Information

States compliance with relevant safety and EMC standards.

Index

Index of Topics and Keywords

Provides an alphabetical index for quick topic lookup within the manual.

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