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General| Type | Fluorescence Spectrophotometer |
|---|---|
| Excitation Source | Xenon Lamp |
| Detector | PMT (Photomultiplier Tube) |
| Spectral Resolution | 1 nm |
| Sample Compartment | Standard cuvette holder; optional accessories available |
Overview of the spectrofluorometer systems and their components.
General safety precautions to be observed during instrument operation.
Detailed information on hazards associated with UV light exposure.
Hazards and precautions related to the use of xenon lamps.
Essential safety training and understanding for instrument users.
Procedures for safely unpacking and setting up the spectrofluorometer system.
Step-by-step guide for removing the instrument from its packaging and initial setup.
Instructions for configuring the host computer system for instrument control.
Steps for establishing the communication link between the instrument and the PC.
Instructions for installing the primary control and data acquisition software.
Explanation of how the spectrofluorometer measures fluorescence.
Information about the host computer and FluorEssence software essential for operation.
Step-by-step procedure for powering on the instrument and associated components.
Routine checks to verify instrument calibration, repeatability, and throughput.
Procedure to verify and adjust the excitation monochromator's wavelength calibration.
Procedure to verify and adjust the emission monochromator's wavelength calibration.
How to select and configure different types of spectral acquisition experiments.
Method for determining optimal excitation and emission wavelengths for new samples.
Procedures and precautions for acquiring spectral data from a sample.
Techniques to enhance data quality by increasing the signal-to-noise ratio.
Method for selecting the appropriate integration time for data acquisition.
How to choose wavelength increments for spectral scans to balance resolution and time.
How to adjust slit widths to control spectral resolution and signal intensity.
Methods for applying correction factors to compensate for instrumental responses.
Ensuring the instrument configuration includes appropriate correction files before data acquisition.
Applying correction factors to acquired data using mathematical operations.
Procedures and precautions for replacing the xenon lamp.
Safety warnings and precautions related to instrument maintenance procedures.
Detailed steps for safely removing and replacing the instrument's lamp.
Procedures for optimizing the new lamp's position for maximum signal output.
Detailed steps and adjustments for optimizing the lamp's position.
Potential causes and solutions for issues with light delivery to the sample.
Troubleshooting steps for low signal output from the instrument.
Possible causes and remedies when signal intensity remains constant unexpectedly.
Troubleshooting steps for situations with no signal or unstable, erratic readings.
Solutions for connectivity issues between the host PC and the spectrofluorometer.
Troubleshooting steps for hardware initialization errors.
Diagnosing and resolving issues with the sample turret's functionality.
Troubleshooting steps for unexpected resets in phosphorimeter mode.
Solutions for errors related to accessing or reading data files.
Procedure to check and configure the system's BIOS settings.
How to interpret spectral data for system diagnosis and troubleshooting.
Using lamp scans to verify system integrity and check parameters.
Using water Raman scans to identify calibration or accessory problems.
Steps to take and information to provide when contacting technical support.
Process for creating or updating emission correction factor files.
Detailed steps for calculating emission correction factors using irradiance data.
Procedure for calculating excitation correction factors using rhodamine-B.
How to configure the software to apply acquired correction factors.
Explanation of the principles behind phosphorescence measurements.
Key parameters governing phosphorimeter experiment setup and data acquisition.
How to generate and analyze phosphorescence decay curves.
