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General| Brand | illumina |
|---|---|
| Model | iSeq 100 |
| Category | Recording Equipment |
| Language | English |
Overview of the Illumina iSeq 100 Sequencing System as a targeted NGS approach.
Links to Illumina website for system support, software, and documentation.
Identification and description of the iSeq 100 system's physical parts and ports.
Details on the single-use reagent kit, its contents, and storage requirements.
Guided process for initial instrument setup, including system checks and settings.
Instructions on minimizing the control software to access other applications.
Configuration options for run setup, monitoring, and data analysis.
Settings for instrument naming, audio, thumbnail images, and software updates.
Configuring WiFi or Ethernet connections for system operation and data transfer.
List of consumables and equipment required from the user for operation.
Overview of the sequencing process stages: cluster generation, sequencing, and analysis.
Step-by-step guide for safely thawing the reagent cartridge before use.
Procedures for diluting libraries and preparing the flow cell for sequencing.
Instructions on loading the flow cell and diluted library into the cartridge.
Guide for setting up a run using the Local Run Manager software.
Guide for setting up a run using manual parameters in the control software.
Steps to delete completed runs and free up disk space on the instrument.
Procedures for installing automatic or manual software updates.
Instructions for replacing the instrument's air filter for proper cooling.
Safe procedures for moving the instrument to a new location.
Information on managing Windows Software Restriction Policies for system security.
Explanation of the Real-Time Analysis software and its role in base calling.
Detailed breakdown of the RTA2 workflow stages: intensity extraction to quality scoring.
Workflow for troubleshooting common error messages during instrument operation.
How to monitor run status and troubleshoot issues in Process Management.
Steps to safely cancel an ongoing sequencing run.
Procedures for safely shutting down and restarting the instrument.
Guide for performing a system check using reusable test components.
Steps to assess and address fluid leaks detected during operation.
Process for resetting the system to factory defaults for software or configuration issues.
Overview of Illumina's advanced exchange program for system issues.
Procedure for receiving and installing a replacement instrument.
Instructions for shutting down, disconnecting, and packaging the original system.
Steps to safely shut down the system and disconnect cables before return.
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