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illumina MiSeq - User Manual

illumina MiSeq
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FOR RESEARCH USE ONLY
ILLUMINA PROPRIETARY
Part # 15027617 Rev. A DRAFT
July 2011
MiSeq
Sequencing System
User Guide

Other manuals for illumina MiSeq

Questions and Answers

  • T
    tammy97Jul 31, 2025
    What to do if illumina MiSeq displays 'Fluidics check failed'?
    • J
      jacob44Jul 31, 2025
      Select 'Pump'. After the fluids have been pumped, select 'Restart Check'.
  • A
    Amanda LawsonAug 5, 2025
    What to do if illumina MiSeq shows 'Network disconnected'?
    • A
      andrewmckenzieAug 5, 2025
      First, ensure the network cable is securely plugged into the instrument. If the network connection issue persists, try rebooting the instrument. Turn off the power switch, wait for at least 60 seconds, and then turn the instrument back on.
  • K
    Kenneth StevensAug 9, 2025
    What to do if illumina MiSeq Recording Equipment shows 'Disk space low'?
    • J
      Jason JonesAug 9, 2025
      If the disk space is low, a message will appear indicating the required disk space and a 'Manage Runs' button. Select 'Manage Runs' and clear the necessary space from the instrument computer.
  • T
    Tanya JohnsonAug 13, 2025
    What to do if Illumina Recording Equipment displays 'Improperly formatted sample sheet'?
    • K
      Kevin LiuAug 13, 2025
      The sample sheet might contain missing information or syntax errors. Correct the errors as listed on the screen, and then select 'Restart Check'.
  • C
    Cynthia BensonAug 18, 2025
    What to do if Illumina Recording Equipment displays 'Sample sheet not found'?
    • F
      Frank RobertsAug 18, 2025
      Check the folder location for the sample sheet in 'Setup Options' on the Welcome screen. If the sample sheet is missing, create one and copy it to the MiSeq. Once completed, select 'Restart Check'.
  • D
    Dawn SheltonAug 22, 2025
    How to fix 'Initialization failed' on illumina MiSeq Recording Equipment?
    • J
      Johnny LinAug 22, 2025
      If initialization failed, an option to re-initialize should appear on the screen. Select 'Re-initialize'.
  • M
    matthew67Aug 27, 2025
    How to empty waste bottle on Illumina Recording Equipment?
    • M
      Mary DavisAug 27, 2025
      Open the reagent compartment door, raise the sipper handle, and empty the waste bottle into an appropriate waste container.

Summary

Getting Started

Components

Details the main physical components of the MiSeq instrument.

Starting the MiSeq

Step-by-step guide on powering on and initializing the MiSeq.

MiSeq Control Software

MiSeq Control Software Interface

Describes the MCS interface, including the Welcome screen and activity indicators.

Performing a Run

Preparing the MiSeq Reagent Cartridge

Provides instructions for thawing and preparing the reagent cartridge.

Setting Up a Run

Guides through editing the sample sheet and loading components.

Post-Run Procedures

Performing a Post-Run Wash

Details the steps for performing a post-run wash.

Shutting Down the Instrument

Provides instructions for safely powering off the instrument.

Troubleshooting

Software Errors

Provides an overview of common software errors and their actions.

illumina MiSeq Specifications

General IconGeneral
Brandillumina
ModelMiSeq
CategoryRecording Equipment
LanguageEnglish

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