MiSeq User Guide
v
Table of Contents
Revision History iii
Table of Contents v
List of Tables vii
Chapter 1 Getting Started 1
Introduction 2
Components 4
Starting the MiSeq 8
MiSeq Workflow 10
Consumables 11
Chapter 2 MiSeq Control Software 13
Introduction 14
MiSeq Control Software Interface 15
Stopping or Pausing a Run 22
Sample Sheets 23
Analysis Workflow 24
Run Folders 25
Available Disk Space 26
Chapter 3 Performing a Run 27
Introduction 28
Preparing the MiSeq Reagent Cartridge 29
Setting Up a Run 31
Monitoring the Run 36
Chapter 4 Post-Run Procedures 37
Introduction 38
Performing a Post-Run Wash 39
Performing a Maintenance Wash 40
Unloading Run Components 42
Shutting Down the Instrument 43
Chapter 5 On-Instrument Analysis 45
Introduction 46
Primary Analysis Results 47
Secondary Analysis Using MiSeq Reporter 49
Chapter 6 Troubleshooting 51
Introduction 52