Getting Started
4
Part # 15027617 Rev. A DRAFT
Components
The MiSeq has the following main components.
} Flow Cell Compartment—Contains the flow cell stage that houses the flow cell
throughout the run. The flow cell stage is controlled by motors that move the stage
out of the enclosed optical module for flow cell loading, and returns it when the run
begins.
} Reagent Compartment—Holds reagents used during runs, wash solutions used
during instrument washes, and the waste bottle. The reagent compartment door is
secured by a magnetic latch.
} Enclosed Optics Module—Contains optical components that enable imaging of the
flow cell.
} Status Bar—Uses three colors to indicate instrument status. Blue indicates the
instrument is running, orange indicates the instrument needs attention, and green
indicates that the instrument is ready to begin the next run.
} Touch Screen Monitor—Enables easy run setup using the software interface and at-
a-glance run monitoring.
Figure 1 Main Compartments
A Flow Cell Compartment
B Enclosed Optics Module
C Status Bar
D Touch Screen Monitor
E Reagent Compartment
The MiSeq software interface guides you through the run setup steps using the touch
screen monitor. To load run components, you need to access the reagent compartment
and the flow cell compartment.
Reagent Compartment
The reagent compartment contains the reagent chiller, and positions for the
incorporation wash buffer (PR2) bottle and the waste bottle.
The reagent chiller holds a single-use MiSeq reagent cartridge during the run, or a wash
cartridge during instrument washes. Depending on the process being performed, the
software automatically lowers sippers into the reagent wells of the cartridge at the
appropriate time in the process.
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