illumina MiSeqDx - User Manual

Questions and Answers

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Alfred McconnellJul 31, 2025
How to troubleshoot flow rate error on illumina MiSeqDx?
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  Joseph JohnsonJul 31, 2025
  The flow rate is the speed in which fluids pass through the fluidics system (µl/min). It is measured before each run during the pre-run check. If the system is unable to measure the flow rate, pump a volume of reagent (MiSeqDx SBS Solution (PR2)) through the system before checking the flow rate again. Use the drop-down list or on-screen keyboard to enter the following information: - Solution: PR2 - Volume: 250 µl - Aspirate Rate: 2500 µl/min - Dispense Rate: 2500 µl/min Select Pump. When the pump step is complete, select Restart Check. If the error persists, set the volume to pump 500 µl MiSeqDx SBS Solution (PR2) and repeat the process 1 more time. Contact Illumina Technical Support if the second attempt does not resolve the error.
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Mr. Troy RichardsonAug 5, 2025
How to resolve flow rate measured issue in illumina Laboratory Equipment?
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  Jason BrownAug 5, 2025
  The flow rate check screen opens. Use the drop-down list or on-screen keyboard to enter the following: • Solution: PR2 • Volume: 250 • Aspirate Rate: 2500 • Dispense Rate: 2500 Select Pump. If the error persists, set the volume to pump 500 µl MiSeqDx SBS Solution (PR2) and repeat the process. When fluids have been pumped, select Restart Check. If the flow check fails again, reseat the flow cell to make sure that flow is not interrupted due to misalignment. Inspect the flow cell gasket for lint or irregularities.
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Sean BridgesAug 9, 2025
How to prevent reboots during a run on MiSeqDx Laboratory Equipment?
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  Ethan ThorntonAug 9, 2025
  If the MiSeqDx Laboratory Equipment restarts during a run, it could be due to Windows Update automatically installing software updates. To prevent this, contact your local IT department to disable automatic updates to the Windows operating system.
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eileendanielsAug 13, 2025
What to do if the sample sheet is not present for illumina MiSeqDx?
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  Kelly MercadoAug 13, 2025
  If the sample sheet is not named with the reagent cartridge ID, the instrument cannot automatically locate it. Browse to the correct sample sheet for the run. If the sample sheet is correctly named, ensure it is in the default sample sheet folder specified in Run Options on the Home screen. Confirm that the file extension is *.csv. If missing, create a sample sheet and copy it to the specified locations. After locating the sample sheet, select Restart Check.
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Selena MillerAug 18, 2025
What to do if free disk space is low on illumina MiSeqDx Laboratory Equipment?
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  Nicholas WilliamsAug 18, 2025
  If disk space is low, a message will appear indicating the required space. Use the Manage Files feature to clear the necessary space from the instrument computer.
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kellychristopherAug 21, 2025
What to do if primary analysis is not ready on illumina MiSeqDx?
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  fmartinezAug 22, 2025
  Primary analysis from the previous run is not complete. The default time to allow primary analysis to complete is 1 hour, and a countdown appears on the screen. You can either wait 1 hour or select Terminate Analysis. Note that secondary analysis stops for any incomplete cycles.
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Stephanie GarciaAug 27, 2025
What to do if network connection is not active on illumina MiSeqDx Laboratory Equipment?
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  Katelyn SmithAug 27, 2025
  Make sure the network cable is properly connected to the instrument. If the connection doesn't restore, reboot the software using the Reboot option on the Manage Instrument screen. If the problem continues, shut down the instrument via the Manage Instrument screen, turn off the power, wait at least 60 seconds, then turn the instrument back on and restart the software.