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NextSeq 550Dx
illumina NextSeq 550Dx User Manual
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Table of Contents
Default Chapter
4
Table of Contents
4
Chapter 1 Overview
6
About this Guide
6
Introduction
6
Additional Resources
7
Instrument Components
7
Sequencing Consumables Overview
11
Chapter 2 Getting Started
16
Starting the Instrument
16
Customize System Settings
17
User-Supplied Consumables and Equipment
18
Chapter 3 Sequencing
20
Introduction
20
Sequencing Workflow
21
Prepare the Reagent Cartridge
21
Prepare the Flow Cell
22
Prepare Libraries for Sequencing
22
Set up a Sequencing Run
23
Monitor Run Progress
29
Automatic Post-Run Wash
31
Chapter 4 Scanning
32
Introduction
32
Scanning Workflow
33
Download the DMAP Folder
33
Load the Beadchip Onto the Adapter
34
Set up a Scan
35
Monitor Scan Progress
37
Chapter 5 Maintenance
40
Introduction
40
Perform a Manual Wash
40
Replace Air Filter
43
Software Updates
44
Reboot and Shut down Options
45
Appendix A Troubleshooting
48
Introduction
48
Troubleshooting Files
48
Resolve Automatic Check Errors
49
Spent Reagents Container Is Full
50
Rehybridization Workflow
51
Beadchip and Scan Errors
53
Custom Recipes and Recipe Folders
54
RAID Error Message
54
Configure System Settings
55
Appendix B Real-Time Analysis
58
Real-Time Analysis Overview
58
Real-Time Analysis Workflow
59
Appendix C Output Files and Folders
64
Sequencing Output Files
64
Sequencing Output Folder Structure
66
Scanning Output Files
67
Scanning Output Folder Structure
68
Index
70
Technical Assistance
74
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illumina NextSeq 550Dx Specifications
General
Brand
illumina
Model
NextSeq 550Dx
Category
Laboratory Equipment
Language
English
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