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illumina NextSeq 550Dx User Manual

illumina NextSeq 550Dx
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NextSeq 550Dx Research Mode
Instrument Reference Guide
Document # 1000000041922 v01
March 2018
ILLUMINA PROPRIETARY
For Research Use Only. Not for use in diagnostic procedures.

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illumina NextSeq 550Dx Specifications

General IconGeneral
Brandillumina
ModelNextSeq 550Dx
CategoryLaboratory Equipment
LanguageEnglish

Summary

Chapter 1 Overview

Sequencing Features

Details on high-throughput sequencing and RTA.

Array Scanning Features

Features related to array scanning capabilities.

Sequencing Consumables Overview

Flow Cell Overview

Reagent Cartridge Overview

Information about the reagent cartridge.

Reagent Cartridge Overview

Chapter 3 Sequencing

Prepare the Reagent Cartridge

Step-by-step instructions for preparing the reagent cartridge.

Set Up a Sequencing Run

Steps to configure and initiate a sequencing run.

Empty the Spent Reagents Container

Procedure for removing and disposing of used reagents.

Load the Buffer Cartridge

Instructions for installing the buffer cartridge.

Enter Run Parameters (Standalone Configuration)

Setting parameters for standalone runs.

Review Pre-Run Check

Automated checks before starting a run.

Sequencing Workflow

Step-by-step guide for performing a sequencing run.

Prepare the Flow Cell

Instructions for preparing the flow cell for sequencing.

Load the Reagent Cartridge

Instructions for installing the reagent cartridge.

Specify Run Parameters

Settings for configuring a sequencing run.

Start the Run

Initiating the sequencing run.

Automatic Post-Run Wash

The automated wash process after a run.

Prepare Libraries for Sequencing

Guidance on denaturing and diluting libraries.

Load the Flow Cell

Instructions for installing the flow cell.

Monitor Run Progress

Tracking the progress and metrics of a sequencing run.

Chapter 2 Getting Started

Starting the Instrument

Instructions for powering on and initializing the instrument.

Scanning

Load the BeadChip Onto the Adapter

Instructions for mounting the BeadChip on the adapter.

Scanning Workflow

Step-by-step guide for performing a scan.

Download the DMAP Folder

Procedure for obtaining DMAP files for scanning.

Set Up a Scan

Steps to configure and initiate a scan.

Monitor Scan Progress

Tracking the progress of a scan.

Chapter 5 Maintenance

Replace Air Filter

Procedure for replacing the instrument's air filter.

Software Updates

Information on installing software updates.

Reboot and Shut Down Options

Procedures for restarting and shutting down the instrument.

Prepare for a Manual Post-Run Wash

Steps to prepare for a manual post-run wash.

Load a Used Flow Cell and the Wash Cartridges

Instructions for loading components for a wash.

Reboot and Shut Down Options

Shut Down the Instrument

Procedure for safely powering off the instrument.

Appendix A Troubleshooting

BeadChip and Scan Errors

Common errors related to BeadChip scanning.

Configure System Settings

Options for configuring network and analysis settings.

Software Cannot Read the BeadChip Barcode

Troubleshooting steps for barcode reading issues.

Perform a Manual Wash

Instructions for performing a manual wash.

Resolve Automatic Check Errors

Actions to resolve pre-run check failures.

Configure System Settings

BeadChip Scan Configuration

Appendix B Real-Time Analysis

Real-Time Analysis Workflow

Base Calling

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