illumina NextSeq 550Dx User Manual

Appendix A Troubleshooting
Introduction 43
Troubleshooting Files 43
Resolve Automatic Check Errors 44
Spent Reagents Container is Full 45
Rehybridization Workflow 46
BeadChip and Scan Errors 48
Custom Recipes and Recipe Folders 49
RAID Error Message 49
Configure System Settings 50
Introduction
For technical questions, visit the NextSeq 550Dx instrument support pages on the Illumina website. The
support pages provide access to documentation, downloads, and frequently asked questions.
Log in to your MyIllumina account for access to support bulletins.
For run quality or performance problems, contact Illumina Technical Support. See Technical Assistance
on page 69.
Consider sharing a link to the run summary in BaseSpace with Illumina Technical Support to facilitate
troubleshooting.
Troubleshooting Files
An Illumina Technical Support representative might request copies of run-specific or scan-specific files
to troubleshoot issues. Typically, the following files are used for troubleshooting.
Troubleshooting Files for Sequencing Runs
Key File Folder Description
Run information file
(RunInfo.xml)
Root
folder
Contains the following information:
• Run name
• Number of cycles in the run
• Number of cycles in each read
• Whether the read is an indexed read
• Number of swaths and tiles on the flow cell
Run parameters file
(RunParameters.xml)
Root
folder
Contains information about run parameters and run components. Information
includes the RFID, serial number, part number, and expiration date.
RTA configuration file
(RTAConfiguration.xml)
Root
folder
Contains the RTA configuration settings for the run.
The RTAConfiguration.xml file is created at the beginning of the run.
InterOp files (*.bin) InterOp Binary reporting files used for Sequencing Analysis Viewer.
InterOp files are updated throughout the run.
Log files Logs Log files describe each step performed by the instrument for each cycle, and list
software and firmware versions used with the run. The file named
[InstrumentName]_CurrentHardware.csv lists the serial numbers of instrument
components.
Error log files
(*ErrorLog*.txt)
RTAlogs Log of RTA errors.
Error log files are updated whenever an error occurs.
Global log files
(*GlobalLog*.tsv)
RTAlogs Log of all RTA events.
Global log files are updated throughout the run.
Document # 1000000041922 v01
For Research Use Only. Not for use in diagnostic procedures.
43

illumina NextSeq 550Dx Specifications

General

Brand	illumina
Model	NextSeq 550Dx
Category	Laboratory Equipment
Language	English