illumina NextSeq 550Dx User Manual

Chapter 5 Maintenance
Introduction 35
Perform a Manual Wash 35
Replace Air Filter 38
Software Updates 39
Reboot and Shut Down Options 40
Introduction
Maintenance procedures include manual instrument washes and system software updates when
available.
u Instrument washes—An automatic post-run wash after each sequencing run maintains instrument
performance. However, a manual wash is required periodically under certain conditions. See Perform
a Manual Wash on page 35.
u Software updates—When an updated version of the system software is available, you can install
the update automatically through a connection to BaseSpace or manually after downloading the
installer from the Illumina website. See Software Updates on page 39.
u Air filter replacement—Regular replacement of the air filter ensures proper air flow through the
instrument.
Preventive Maintenance
Illumina recommends that you schedule a preventive maintenance service each year. If you are not
under a service contract, contact your Territory Account Manager or Illumina Technical Support to
arrange for a billable preventive maintenance service.
Perform a Manual Wash
Manual washes are initiated from the Home screen. Wash options include the Quick Wash and the
Manual Post-Run Wash.
Wash Types Description
Quick Wash
Duration: 20 minutes
Flushes the system with a user-supplied wash solution of laboratory-grade water and
Tween 20 (buffer wash cartridge).
• Required every 14 days that the instrument is idle with reagent cartridge and buffer
cartridge in place.
• Required every 7days that the instrument is in a dry state (reagent cartridge and
buffer cartridge removed).
• Required after a shutdown.
Manual Post-Run Wash
Duration: 90 minutes
Flushes the system with a user-supplied wash solution of laboratory-grade water and
Tween 20 (buffer wash cartridge) and 0.12% sodium hypochlorite(reagent wash
cartridge).
Required if the automatic post-run wash was not performed.
A manual wash requires the reagent wash cartridge and buffer wash cartridge provided with the
instrument, and a used flow cell. A used flow cell can be used up to 20 times for instrument washes.
Document # 1000000041922 v01
For Research Use Only. Not for use in diagnostic procedures.
35

illumina NextSeq 550Dx Specifications

General

Brand	illumina
Model	NextSeq 550Dx
Category	Laboratory Equipment
Language	English