Figure 25 Reagent Wash Cartridge and Buffer Wash Cartridge
Prepare for a Manual Post-Run Wash
Choose to either prepare for a manual post-run wash as described below, or prepare for a quick wash
(next section). If you intend to do a manual post-run wash, skip the quick wash section and continue on
to
Load a Used Flow Cell and the Wash Cartridges
on page 37.
User-Supplied
Consumables
Volume and Description
NaOCl 1 ml, diluted to 0.12%
Loaded onto the reagent wash cartridge (position #28)
100% Tween 20
Laboratory-grade water
Used to make 125 ml 0.05% Tween 20 wash solution
Loaded onto the buffer wash cartridge (center reservoir)
NOTE
Always use a fresh dilution of NaOCl prepared within the last 24hours. If you make a volume larger
than 1ml, store the remaining dilution at 2°C to 8°C for use within the next 24 hours. Otherwise,
discard the remaining dilution of NaOCl.
1 Combine the following volumes in a microcentrifuge tube to result in 1 ml 0.12% NaOCl:
u 5% NaOCl (24µl)
u Laboratory-grade water (976 µl)
2 Invert the tube to mix.
3 Add 1 ml of 0.12% NaOCl to the reagent wash cartridge. The correct reservoir is equivalent to
position #28 on the prefilled cartridge.
Figure 26 Load NaOCl
4 Combine the following volumes to result in a 0.05% Tween 20 wash solution:
u 100% Tween 20 (62 µl)
Document # 1000000041922 v01
For Research Use Only. Not for use in diagnostic procedures.
36
NextSeq 550Dx Research Mode Instrument Reference Guide
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