NOTE
If more than one cartridge is thawing in the same water bath, allow additional thawing time.
3 Invert the cartridge 5 times to mix reagents.
4 Inspect the bottom of the cartridge to make sure that reagents are thawed and free of precipitates.
Confirm that positions 29, 30, 31, and 32 are thawed, as they are the largest and will take the
longest to thaw.
5 Gently tap on the bench to reduce air bubbles.
For best results, proceed directly to loading the sample and setting up the run.
WARNING
This set of reagents contains potentially hazardous chemicals. Personal injury can occur
through inhalation, ingestion, skin contact, and eye contact. Wear protective equipment,
including eye protection, gloves, and laboratory coat appropriate for risk of exposure. Handle
used reagents as chemical waste and discard in accordance with applicable regional, national,
and local laws and regulations. For additional environmental, health, and safety information, see the
SDS at support.illumina.com/sds.html.
Prepare the Flow Cell
1 Remove a new flow cell package from 2°C to 8°C storage.
2 Set the unwrapped flow cell package aside at room temperature for 30 minutes.
NOTE
If the foil package is intact, the flow cell can remain at room temperature up to 12 hours. Avoid
repeated cooling and warming of the flow cell.
Prepare Libraries for Sequencing
Denature and Dilute Libraries
Denature and dilute your libraries to a loading volume of 1.3 ml and a loading concentration of 1.8pM. In
practice, loading concentration can vary depending on library preparation and quantification methods. For
instructions, see the
NextSeq System Denature and Dilute Libraries Guide (document # 15048776)
.
Load Libraries onto the Reagent Cartridge
1 Clean the foil seal covering reservoir #10 labeled Load Library Here using a low-lint tissue.
2 Pierce the seal with a clean 1 ml pipette tip.
3 Load 1.3 ml of prepared 1.8 pM libraries into reservoir #10 labeled Load Library Here. Avoid
touching the foil seal as you dispense the libraries.
Document # 1000000041922 v01
For Research Use Only. Not for use in diagnostic procedures.
17
NextSeq 550Dx Research Mode Instrument Reference Guide
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