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illumina NextSeq 500 User Manual

illumina NextSeq 500
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NextSeq 500
System Guide
Document # 15046563 v04
May 2018
ILLUMINA PROPRIETARY
For Research Use Only. Not for use in diagnostic procedures.
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illumina NextSeq 500 Specifications

General IconGeneral
Sequencing TechnologySequencing by Synthesis (SBS)
Power Requirements100-240 VAC, 50/60 Hz
Maximum Samples per Runup to 96 samples
Outputup to 120 Gb
Sample TypeDNA and RNA
ApplicationsTargeted Sequencing
ReadsUp to 400 million

Summary

Chapter 1 Overview

Chapter 2 Getting Started

Starting the Instrument

Step-by-step instructions for powering on and initializing the NextSeq 500 system.

Chapter 3 Sequencing

Sequencing Workflow Steps

Step-by-step guide for preparing and initiating a sequencing run, from cartridge prep to monitoring.

Preparing the Reagent Cartridge

Instructions for thawing, inspecting, and preparing the reagent cartridge for use.

Preparing the Flow Cell

Guidance on preparing the flow cell, including bringing it to room temperature and cleaning.

Library Preparation and Loading

How to denature, dilute, and load libraries onto the reagent cartridge for sequencing.

Setting Up and Starting a Sequencing Run

Steps to configure and start a sequencing run via the software interface and automated checks.

Chapter 4 Maintenance

Manual Wash Procedures

Instructions for performing manual washes: Quick Wash and Manual Post-Run Wash.

System Software Updates

Guide for installing automatic or manual software updates for the system suite.

Appendix A Troubleshooting

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