illumina NextSeq 500 - User-Supplied Consumables and Equipment

Set Automatic Purge Option
1 From the Manage Instrument screen, select System Customization.
2 Select Save to advance to Run Customization.
3 Select the Purge Consumables at End of Run checkbox to purge unused reagents from the reagent
cartridge to the spent reagents container automatically after each run.
NOTE
Purging consumables automatically adds additional time to the workflow.
4 Select Save to save settings and exit the screen.
User-Supplied Consumables and Equipment
The following consumables and equipment are used on the NextSeq 500.
User-Supplied Consumables for Sequencing Runs
Consumable Supplier Purpose
1 N NaOH
(sodium hydroxide)
General lab supplier Library denaturation, diluted to 0.2N
200 mM Tris-HCl, pH7 General lab supplier Library denaturation
Alcohol wipes, 70% Isopropyl
or
Ethanol, 70%
VWR, catalog#95041-714
(or equivalent)
General lab supplier
Flow cell cleaning and general purpose
Lab tissue, low-lint VWR, catalog#21905-026
(or equivalent)
Flow cell cleaning
User-Supplied Consumables for Instrument Maintenance
Consumable Supplier Purpose
NaOCl, 5%
(sodium hypochlorite)
Sigma-Aldrich, catalog # 239305
(or laboratory-grade equivalent)
Washing the instrument using the
manual post-run wash; diluted to 0.12%
Tween 20 Sigma-Aldrich, catalog # P7949 Washing the instrument using manual
wash options; diluted to 0.05%
Water, laboratory-grade General lab supplier Washing the instrument (manual wash)
Guidelines for Laboratory-Grade Water
Always use laboratory-grade water or deionized water to perform instrument procedures. Never use tap
water. Use only the following grades of water or equivalents:
u Deionized water
u Illumina PW1
u 18Megohms (MΩ) water
u Milli-Q water
u Super-Q water
u Molecular biology grade water
Document # 15046563 v04
For Research Use Only. Not for use in diagnostic procedures.
10
NextSeq 500 System Guide