Thumbnail Image Naming
A thumbnail image for each color channel (red and green) for tiles 1, 6, and 12 are generated from all
cameras, top and bottom surfaces, at every cycle during imaging. Thumbnail files are generated in the JPG
file format.
Each image is named with the tile number as indicated by the following naming convention, which always
begins with s_:
u Lane—1, 2, 3, or 4
u Tile—A 5-digit tile number, which indicates surface, swath, camera, and tile
u Channel—Red or green
Example: s_3_12512_green.jpg, which indicates lane 3, top surface, swath 2, camera 5, tile 12, and the
green channel.
Output Folder Structure
The control software generates the output folder name automatically.
Data
Intensities
BaseCalls
L001—Base call files for lane 1, aggregated in 1 file per cycle.
L002—Base call files for lane 2, aggregated in 1 file per cycle.
L003—Base call files for lane 3, aggregated in 1 file per cycle.
L004—Base call files for lane 4, aggregated in 1 file per cycle.
L001—An aggregated *.locs file for lane 1.
L002—An aggregated *.locs file for lane 2.
L003—An aggregated *.locs file for lane 3.
L004—An aggregated *.locs file for lane 4.
Images
Focus
L001—Focus images for lane 1.
L002—Focus images for lane 2.
L003—Focus images for lane 3.
L004—Focus images for lane 4.
InterOp—Binary files used by Sequencing Analysis Viewer (SAV).
Logs—Log files describing operational steps.
Recipe—Run-specific recipe file named with reagent cartridge ID.
RTALogs—Log files describing analysis steps.
Thumbnail_Images—Thumbnail images for tiles 1, 6, and 12 in each swath at every cycle.
RTAComplete.xml
Document # 15046563 v04
For Research Use Only. Not for use in diagnostic procedures.
45
NextSeq 500 System Guide
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