illumina NextSeq 500 - Appendix A Troubleshooting; Introduction to Troubleshooting

Appendix A Troubleshooting
Introduction 29
Troubleshooting Files 29
Resolve Automatic Check Errors 30
Spent Reagents Container is Full 31
Rehybridization Workflow 31
Custom Recipes and Recipe Folders 33
System Check 33
RAID Error Message 35
Configure System Settings 35
Introduction
For technical questions, visit the NextSeq 500 support pages on the Illumina website. The support pages
provide access to documentation, downloads, and frequently asked questions.
Log in to your MyIllumina account for access to support bulletins.
For run quality or performance problems, contact Illumina Technical Support. See Technical Assistance on
page 50.
Consider sharing a link to the run summary in BaseSpace with Illumina Technical Support to facilitate
troubleshooting.
Troubleshooting Files
An Illumina Technical Support representative might request copies of run-specific or scan-specific files to
troubleshoot issues. Typically, the following files are used for troubleshooting.
Key File Folder Description
Run information file
(RunInfo.xml)
Root folder Contains the following information:
• Run name
• Number of cycles in the run
• Number of cycles in each read
• Whether the read is an indexed read
• Number of swaths and tiles on the flow cell
Run parameters file
(RunParameters.xml)
Root folder Contains information about run parameters and run components.
Information includes the RFID, serial number, part number, and expiration
date.
RTA configuration file
(RTAConfiguration.xml)
Data\Intensities Contains the RTA configuration settings for the run.
The RTAConfiguration.xml file is created at the beginning of the run.
InterOp files (*.bin) InterOp Binary reporting files used for Sequencing Analysis Viewer.
InterOp files are updated throughout the run.
Log files Logs Log files describe each step performed by the instrument for each cycle,
and list software and firmware versions used with the run. The file named
[InstrumentName]_CurrentHardware.csv lists the serial numbers of
instrument components.
Error log files
(*ErrorLog*.txt)
RTALogs Log of RTA errors.
Error log files are updated whenever an error occurs.
Global log files
(*GlobalLog*.tsv)
RTALogs Log of all RTA events.
Global log files are updated throughout the run.
Lane log files
(*LaneLog*.txt)
RTALogs Log RTA processing events.
Lane log files are updated throughout the run.
Document # 15046563 v04
For Research Use Only. Not for use in diagnostic procedures.
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