Power Button
The power button on the front of the NextSeq turns on power to the instrument and instrument computer.
The power button performs the following actions depending on the state of instrument power.
Power State Action
Instrument power is
off
Briefly press the button to turn on the power.
Instrument power is
on
Briefly press the button to turn off the power. A dialog box appears on the screen to confirm a normal
instrument shutdown.
Instrument power is
on
Press and hold the power button for 10 seconds to cause a hard shutdown of the instrument and
instrument computer.
Use this method to turn off the instrument only if the instrument is unresponsive.
NOTE
Turning off the instrument during a sequencing run ends the run immediately. Ending a run is final. Run
consumables cannot be reused and sequencing data from the run is not saved.
Sequencing Consumables Overview
Contents and Storage
The sequencing consumables required to run the NextSeq are provided separately in a single-use kit. Each
kit includes one flow cell, a reagent cartridge, a buffer cartridge, and library dilution buffer. When you receive
NextSeq 500 Kit:
u Do not open the white foil packages until instructed to do so.
u Promptly store components at the indicated temperatures to ensure proper performance.
u Store cartridges so that the package labels face up.
Consumable Quantity Storage Temperature Description
Reagent Cartridge 1 -25°C to -15°C Contains clustering and sequencing reagents
Buffer Cartridge 1 15°C to 30°C Contains buffer and wash solution
HT1 1 -25°C to -15°C Hybridization Buffer
Flow Cell 1 2°C to 8°C* Single-use flow cell
*Shipped at room temperature for NextSeq v2.5 Reagents kits
Reagents are sensitive to light. Store the reagent cartridge and buffer cartridge in a dark location away from
light.
The flow cell, reagent cartridge, and buffer cartridge use radio-frequency identification (RFID) for accurate
consumable tracking and compatibility.
All other kits include dual-index sequencing primers and NaOCIin the prefilled cartridge. No additional steps
are required.
Kit Compatibility Labeling
Kit components are labeled with color-coded indicators to show compatibility between flow cells and reagent
cartridges. Always use a compatible reagent cartridge and flow cell. The buffer cartridge is universal.
Document # 15046563 v04
For Research Use Only. Not for use in diagnostic procedures.
4
NextSeq 500 System Guide
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