Each flow cell and reagent cartridge is labeled High or Mid. Always check the label when you prepare
consumables for a run.
Kit Type Marking on Label
High Output Kit Components
Mid Output Kit Components
Flow Cell Overview
Figure 3 Flow Cell Cartridge
A Lane pair A—Lanes 1 and 3
B Lane pair B—Lanes 2 and 4
C Flow cell cartridge frame
The flow cell is a glass-based substrate on which clusters are generated and the sequencing reaction is
performed. The flow cell is encased in a flow cell cartridge.
The flow cell contains 4 lanes that are imaged in pairs.
u Lanes 1 and 3 (lane pair A) are imaged at the same time.
u Lanes 2 and 4 (lane pair B) are imaged when imaging of lane pair A is complete.
Although the flow cell has 4 lanes, only a single library or set of pooled libraries is sequenced on the flow cell.
Libraries are loaded onto the reagent cartridge from a single reservoir and transferred automatically to the
flow cell to all 4 lanes.
Each lane is imaged in small imaging areas called tiles. For more information, see
Flow Cell Tiles
on page 42.
Document # 15046563 v04
For Research Use Only. Not for use in diagnostic procedures.
5
NextSeq 500 System Guide
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