illumina NextSeq 500 User Manual

Table of Contents
Chapter 1 Overview 1
Introduction 1
Additional Resources 1
Instrument Components 2
Sequencing Consumables Overview 4
Chapter 2 Getting Started 8
Starting the Instrument 8
Customize System Settings 8
Customize Run Settings 9
User-Supplied Consumables and Equipment 10
Chapter 3 Sequencing 12
Introduction 12
Sequencing Workflow 13
Prepare the Reagent Cartridge 13
Prepare the Flow Cell 14
Prepare Libraries for Sequencing 15
Set Up a Sequencing Run 15
Monitor Run Progress 21
Automatic Post-Run Wash 23
Chapter 4 Maintenance 24
Introduction 24
Perform a Manual Wash 24
Software Updates 26
Shut Down the Instrument 27
Appendix A Troubleshooting 29
Introduction 29
Troubleshooting Files 29
Resolve Automatic Check Errors 30
Spent Reagents Container is Full 31
Rehybridization Workflow 31
Custom Recipes and Recipe Folders 33
System Check 33
RAID Error Message 35
Configure System Settings 35
Appendix B Real-Time Analysis 38
Real-Time Analysis Overview 38
Real-Time Analysis Workflow 39
Sequencing Output Files 42
Document # 15046563 v04
For Research Use Only. Not for use in diagnostic procedures.
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illumina NextSeq 500 Specifications

General

Brand	illumina
Model	NextSeq 500
Category	Laboratory Equipment
Language	English