Load a Used Flow Cell and the Wash Cartridges
1 If a used flow cell is not present, load a used flow cell. Select Load, and then select Next.
2 Remove the spent reagents container and discard the contents in accordance with applicable
standards.
WARNING
This set of reagents contains potentially hazardous chemicals. Personal injury can occur through
inhalation, ingestion, skin contact, and eye contact. Wear protective equipment, including eye
protection, gloves, and laboratory coat appropriate for risk of exposure. Handle used reagents as
chemical waste and discard in accordance with applicable regional, national, and local laws and
regulations. For additional environmental, health, and safety information, see the SDS at
support.illumina.com/sds.html.
3 Slide the empty spent reagents container into the buffer compartment until it stops.
4 Remove the used buffer cartridge from the previous run, if present.
5 Load the buffer wash cartridge containing wash solution.
6 Remove the used reagent cartridge from the previous run, if present.
7 Load the reagent wash cartridge.
8 Select Next. The prewash check begins automatically.
Start the Wash
1 Select Start.
2 When the wash is complete, select Home.
After the Wash
After the wash, the sippers remain in the down position to prevent air from entering the system. Leave the
cartridges in place until the next run.
Software Updates
Software updates are packaged in a software bundle called the System Suite, which includes the following
software:
u NextSeq Control Software (NCS)
u NextSeq recipes
u RTA2
u NextSeq Service Software (NSS)
u Sequencing Analysis Viewer (SAV)
u BaseSpace Broker
You can install software updates automatically using an internet connection or manually from a network or
USB location.
u Automatic updates—For instruments connected to a network with internet access, an alert icon
appears on the Manage Instrument button on the Home screen when an update is available.
Document # 15046563 v04
For Research Use Only. Not for use in diagnostic procedures.
26
NextSeq 500 System Guide
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