RTA Errors
To troubleshoot RTA errors, first check the RTA error log, which is stored in the RTALogs folder. This file is not
present for successful runs. Include the error log when reporting issues to Illumina Technical Support.
Resolve Automatic Check Errors
If errors occur during the automatic check, use the following recommended actions to resolve the error.
If a pre-run check fails, the reagent cartridge RFID is not locked and can be used for a subsequent run.
However, the RFID is locked after the foil seals have been pierced.
System Checks Recommended Action
Doors Closed Make sure that the compartment doors are closed.
Consumables Loaded Consumable sensors do not register. Make sure that each consumable is properly
loaded.
On the run setup screens, select Back to return to the loading step, and repeat run
setup.
Required Software Critical components of the software are missing.
Perform a manual software update to restore all software components.
Instrument Disk Space The instrument hard drive does not have sufficient disk space to perform a run. It is
possible that data from a previous run did not transfer.
Clear run data from the instrument hard drive.
Network Connection The network connection has been interrupted. Check network status and the physical
network connection.
Network Disk Space Either the BaseSpace account is full or the network server is full.
Temperature Recommended Action
Temperature Contact Illumina Technical Support.
Temperature Sensors Contact Illumina Technical Support.
Fans Contact Illumina Technical Support.
Imaging System Recommended Action
Imaging Limits Contact Illumina Technical Support.
Z Steps-and-Settle Contact Illumina Technical Support.
Bit Error Rate Contact Illumina Technical Support.
Flow Cell Registration It is possible that the flow cell is not properly seated.
• On the run setup screens, select Back to return to the flow cell step. The imaging
compartment door opens.
• Unload and reload the flow cell to make sure that it is seated properly.
Reagent Delivery Recommended Action
Valve Response Contact Illumina Technical Support.
Pump Contact Illumina Technical Support.
Buffer Mechanism Contact Illumina Technical Support.
Spent Reagents Empty Empty the spent reagents container and reload the empty container.
Document # 15046563 v04
For Research Use Only. Not for use in diagnostic procedures.
30
NextSeq 500 System Guide
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