2 Thaw in a room temperature water bath untilthawed (~60 minutes). Do not submerge the cartridge.
3 Gently tap on the bench to dislodge water from the base, and then dry the base.
NOTE
[Alternate method] Thaw reagents overnight at 2°C to 8°C. Reagents require a minimum of 18 hours to
thaw. At this temperature, reagents are stable up to 1 week.
4 Invert the cartridge 5 times to mix reagents.
5 Inspect positions 29, 30, 31, and 32 to make sure that reagents are thawed.
6 Gently tap on the bench to reduce air bubbles.
WARNING
This set of reagents contains potentially hazardous chemicals. Personal injury can occur through
inhalation, ingestion, skin contact, and eye contact. Wear protective equipment, including eye
protection, gloves, and laboratory coat appropriate for risk of exposure. Handle used reagents as
chemical waste and discard in accordance with applicable regional, national, and local laws and
regulations. For additional environmental, health, and safety information, see the SDS at
support.illumina.com/sds.html.
Prepare the Flow Cell
1 Remove a new flow cell package from 2°C to 8°C storage.
2 Set the unwrapped flow cell package aside at room temperature for 30 minutes.
NOTE
If the foil package is intact, the flow cell can remain at room temperature up to 12 hours. Avoid repeated
cooling and warming of the flow cell.
3 Remove the flow cell from the foil package.
Figure 9 Remove from Foil Package
4 Open the clear plastic clamshell package and remove the flow cell.
Document # 15046563 v04
For Research Use Only. Not for use in diagnostic procedures.
14
NextSeq 500 System Guide
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