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General| Instrument Type | Benchtop Sequencer |
|---|---|
| Sequencing Technology | Sequencing by Synthesis (SBS) |
| Read Length | Up to 2 x 150 bp |
| Output | Up to 7.5 Gb |
| Run Time | 4 - 24 hours |
| Data Quality | ≥ 80% bases ≥ Q30 |
| Maximum Reads | 25 million |
| Sample Capacity | 1 |
| Applications | Targeted Gene Sequencing, Small Genome Sequencing, Amplicon Sequencing, Gene Expression Profiling |
| Power Requirements | 100-240 V, 50-60 Hz |
| Operating Temperature | 19°C to 25°C |
| Operating Humidity | Non-condensing, 20% to 80% RH |
Describes the main physical and software components of the MiniSeq instrument.
Introduces the various consumables required for sequencing runs.
Explains the reagent cartridge, its contents, and usage.
Provides step-by-step instructions for powering on and starting the instrument.
Guides users through configuring various system preferences and options.
Explains options for data transfer and downstream analysis configurations.
Details the steps for setting up and running a sequencing workflow in standalone mode.
Outlines the workflow for runs configured with BaseSpace or Local Run Manager.
Guides on preparing reagents and flow cells for a sequencing run.
Instructions for loading prepared libraries into the reagent cartridge.
Step-by-step guide for properly loading the flow cell into the instrument.
Guides users on specifying run name, library ID, and read parameters.
Covers the process of defining run parameters and initiating a sequencing run.
Instructions for loading the reagent cartridge into the instrument.
Details procedures for performing quick and manual post-run washes.
Details the process for automatically updating the instrument software.
Provides instructions for manually updating the instrument software.
Provides steps to resolve errors detected during the pre-run check.
Addresses issues related to instrument hardware components and performance.
Guides users on initiating and executing a system check on the instrument.
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