illumina MiniSeq User Manual

Workflow (BaseSpace or Local Run Manager Configuration)
Specify run parameters using BaseSpace, BaseSpace Onsite, or Local Run Manager.
Prepare a new reagent cartridge: thaw and inspect.
Prepare a new flow cell: bring to room temperature, unwrap, and inspect.
Denature and dilute libraries. See MiniSeq System Denature and Dilute Libraries Guide (document #
1000000002697) .
Load the library dilution onto the reagent cartridge in reservoir #16.
From the software interface, select Sequence to begin the run setup steps.
Select the run name specified on BaseSpace, BaseSpace Onsite, or Local Run Manager.
Load the flow cell.
Load the reagent cartridge.
Empty and reload the spent reagents bottle.
Review run parameters on the Run Setup screen.
After the automated check, select Start.
Monitor the run on the instrument screen, from BaseSpace or from a networked computer using
Sequencing Analysis Viewer. Data are transferred to BaseSpace, BaseSpace Onsite, or the specified
output folder.
An instrument wash begins automatically when sequencing is complete.
Document # 1000000002695 v02 Material # 20014309
For Research Use Only. Not for use in diagnostic procedures.
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MiniSeq System Guide

illumina MiniSeq Specifications

General

Instrument Type	Benchtop Sequencer
Sequencing Technology	Sequencing by Synthesis (SBS)
Read Length	Up to 2 x 150 bp
Output	Up to 7.5 Gb
Run Time	4 - 24 hours
Data Quality	≥ 80% bases ≥ Q30
Maximum Reads	25 million
Sample Capacity	1
Applications	Targeted Gene Sequencing, Small Genome Sequencing, Amplicon Sequencing, Gene Expression Profiling
Power Requirements	100-240 V, 50-60 Hz
Operating Temperature	19°C to 25°C
Operating Humidity	Non-condensing, 20% to 80% RH