illumina MiniSeq User Manual

Index
2
2-channel imaging 50
A
advanced loading option 9
analysis
output files 52
software 4
analysis configuration 20
analysis, primary
signal purity 50
B
base call files 52
base calling
2-channel 50
indexing considerations 51
BaseSpace 1
login 24
transfer icons 29
BaseSpace Broker 30
BaseSpace configuration 24
C
chastity filter 50
cluster location
files 52
template generation 49
clusters passing filter 50
compatibility
flow cell, reagent cartridge 5
RFID tracking 5-6
components
flow cell compartment 2
imaging compartment 2
reagent compartment 2
status bar 2
configuration settings 45
consumables 5
flow cell 5
instrument maintenance 14
laboratory-grade water 14
reagent cartridge 6
sequencing runs 13
user-supplied 13
wash consumables 32-33
control software 4
customer support 57
cycles in a read 16
D
data transfer
activity icons 29
BaseSpace Broker 30
run copy service 29
databases, preinstalled 7
dbSNP database 7
Demo Mode 9
documentation 1, 57
E
empirical phasing 49
errors
probability 51
errors and warnings 4
in output files 40
F
filter files 52
flow cell
overview 5
preparation 19
rehybridization 41
types 1
flow cell clamp 2
flow cell compartment 2
folder location 23
formamide, position 6 30
H
hard drive space 40
help, technical 57
I
icons
errors and warnings 4
status 4
Illumina Proactive monitoring service 11-13
Document # 1000000002695 v02 Material # 20014309
For Research Use Only. Not for use in diagnostic procedures.
54

illumina MiniSeq Specifications

General

Instrument Type	Benchtop Sequencer
Sequencing Technology	Sequencing by Synthesis (SBS)
Read Length	Up to 2 x 150 bp
Output	Up to 7.5 Gb
Run Time	4 - 24 hours
Data Quality	≥ 80% bases ≥ Q30
Maximum Reads	25 million
Sample Capacity	1
Applications	Targeted Gene Sequencing, Small Genome Sequencing, Amplicon Sequencing, Gene Expression Profiling
Power Requirements	100-240 V, 50-60 Hz
Operating Temperature	19°C to 25°C
Operating Humidity	Non-condensing, 20% to 80% RH