illumina MiniSeq User Manual

6 [Optional] If you are using custom primers, select the checkbox for the primers used.
u Read 1—Custom primer for Read 1.
u Index 1—Custom primer for Index 1.
u Index 2—Custom primer for Index 2.
u Read 2—Custom primer for Read 2.
7 [Optional] Select Advanced Settings to change settings for the current run.
u Purge consumables for this run—Change the setting to purge consumables automatically after the
current run.
u Use run monitoring for this run—Change the setting to use run monitoring in BaseSpace. When
enabled, a BaseSpace login and internet connection are required.
u Recipe—Select a custom recipe for this run.
u Output folder—Change the output folder location for the current run. Select Browse to navigate to a
folder location.
8 Select Next.
NOTE
Do not open the reagent compartment door or the flow cell compartment door during the automated
check or during the sequencing run.
Review Automated Check
1 Review the automated check results.
u To stop a check in progress, select Cancel.
u For any items that do not pass, an action is required before you can proceed. See Automatic Check
Errors on page 39.
u To restart the check, select Retry. The check resumes at the first incomplete or failed check.
2 To start the run, select from the following options.
u If the system is not configured to start automatically after a successful check, select Start.
u If the system is configured to start automatically after a successful check, the sequencing run begins
automatically. You do not have to be present. However, if any errors occur during the check, the run
does not begin automatically.
Set Up a Run (BaseSpace or Local Run Manager Configuration)
1 From the Home screen, select Sequence.
The Sequence command releases consumables from a previous run and opens the series of run setup
screens.
Log in to BaseSpace or Local Run Manager
1 Enter your user name and password.
2 Select Next.
Select Available Run
1 Select a run name from the list of available runs.
Use the up and down arrows to scroll through the list or enter a run name in the Search field.
2 Select Next.
Document # 1000000002695 v02 Material # 20014309
For Research Use Only. Not for use in diagnostic procedures.
24
MiniSeq System Guide

illumina MiniSeq Specifications

General

Instrument Type	Benchtop Sequencer
Sequencing Technology	Sequencing by Synthesis (SBS)
Read Length	Up to 2 x 150 bp
Output	Up to 7.5 Gb
Run Time	4 - 24 hours
Data Quality	≥ 80% bases ≥ Q30
Maximum Reads	25 million
Sample Capacity	1
Applications	Targeted Gene Sequencing, Small Genome Sequencing, Amplicon Sequencing, Gene Expression Profiling
Power Requirements	100-240 V, 50-60 Hz
Operating Temperature	19°C to 25°C
Operating Humidity	Non-condensing, 20% to 80% RH