illumina MiniSeq User Manual

Table of Contents
Chapter 1 Overview 1
Introduction 1
Additional Resources 1
Instrument Components 2
Sequencing Consumables Overview 5
Preinstalled Databases and Genomes 7
Chapter 2 Getting Started 8
Starting the Instrument 8
Customize System Settings 8
Configure Analysis Settings 10
User-Supplied Consumables and Equipment 13
Chapter 3 Sequencing 16
Introduction 16
Sequencing Workflow 17
Prepare Consumables 19
Prepare Libraries for Sequencing 20
Set Up a Sequencing Run 20
Monitor Run Progress 28
Automatic Post-Run Wash 30
Remove Used Reservoir from Position #9 30
Chapter 4 Maintenance 32
Introduction 32
Perform a Manual Instrument Wash 32
Software Updates 35
Appendix A Troubleshooting 38
Troubleshooting Files 38
Automatic Check Errors 39
RTA Errors 40
Rehybridization Workflow 41
System Check 42
Network Configuration Settings 45
Custom Genomes 46
Shut Down the Instrument 46
Appendix B Real-Time Analysis 48
Real-Time Analysis Overview 48
Input and Output Files 48
Real-Time Analysis Workflow 49
Document # 1000000002695 v02 Material # 20014309
For Research Use Only. Not for use in diagnostic procedures.
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illumina MiniSeq Specifications

General

Instrument Type	Benchtop Sequencer
Sequencing Technology	Sequencing by Synthesis (SBS)
Read Length	Up to 2 x 150 bp
Output	Up to 7.5 Gb
Run Time	4 - 24 hours
Data Quality	≥ 80% bases ≥ Q30
Maximum Reads	25 million
Sample Capacity	1
Applications	Targeted Gene Sequencing, Small Genome Sequencing, Amplicon Sequencing, Gene Expression Profiling
Power Requirements	100-240 V, 50-60 Hz
Operating Temperature	19°C to 25°C
Operating Humidity	Non-condensing, 20% to 80% RH