illumina MiniSeq User Manual

If data transfer is interrupted during the run, data are stored temporarily on the instrument computer. When
the connection is restored, data transfer resumes automatically during the run. If the connection is not
restored before the run ends, manually move data to the preferred location.
Transfer to BaseSpace
The BaseSpace Broker transfers run data to BaseSpace. If the connection to BaseSpace is interrupted, the
broker attempts to upload data for 7 days. If the connection is not restored after 7 days, run data are
deleted.
If you specify an additional location for run data, data are transferred to that location regardless of broker
status.
Automatic Post-Run Wash
When the sequencing run is complete, the software initiates an automatic post-run wash using the wash
solution and NaOCl provided in the reagent cartridge.
The automatic post-run wash takes approximately 60 minutes. When the wash is complete, the Home button
becomes active. Sequencing results remain visible on the screen during the wash.
After the Wash
After the wash, the sippers remain in the down position to prevent air from entering the system. Leave the
cartridge in place until the next run.
Remove Used Reservoir from Position #9
The reservoir in position #9 of the reagent cartridge contains formamide. Before discarding the used reagent
cartridge, you can remove the reservoir from position #9 to discard it separately.
1 Wearing gloves, press downwards on the white breakaway tab at position #9 to break the 3 connection
points.
Figure 24 Breakaway Tab at Position #9
A Breakaway tab shown intact
B Reservoir clip
2 Slide the breakaway tab sideways towards the left edge of the cartridge, so that the breakaway tab
slides under the cartridge cover.
Document # 1000000002695 v02 Material # 20014309
For Research Use Only. Not for use in diagnostic procedures.
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MiniSeq System Guide

illumina MiniSeq Specifications

General

Instrument Type	Benchtop Sequencer
Sequencing Technology	Sequencing by Synthesis (SBS)
Read Length	Up to 2 x 150 bp
Output	Up to 7.5 Gb
Run Time	4 - 24 hours
Data Quality	≥ 80% bases ≥ Q30
Maximum Reads	25 million
Sample Capacity	1
Applications	Targeted Gene Sequencing, Small Genome Sequencing, Amplicon Sequencing, Gene Expression Profiling
Power Requirements	100-240 V, 50-60 Hz
Operating Temperature	19°C to 25°C
Operating Humidity	Non-condensing, 20% to 80% RH