illumina MiniSeq User Manual

Resource Description
MiniSeq System Denature
and Dilute Libraries Guide
(document #
1000000002697)
Provides instructions for denaturing and diluting prepared libraries for a sequencing run,
and preparing an optional PhiX control.
Local Run Manager
Software Guide (document
# 1000000002702)
Provides information about using the Local Run Manager software and available analysis
options.
Instrument Components
The MiniSeq System includes a touch screen monitor, a status bar, a flow cell compartment, and a reagent
compartment.
Figure 1 Instrument Components
A Touch screen monitor—Enables on-instrument configuration and setup using the control software interface.
B Power button–Turns on the integrated instrument computer and operating system.
C USB ports—Convenient connections for peripheral components.
D Flow cell compartment—Holds the flow cell during a sequencing run.
E Status bar—Indicates instrument status as processing (blue), requires attention (orange), ready to sequence
(green), or when a wash is due within the next 24 hours (yellow).
F Reagent compartment—Holds the reagent cartridge and spent reagents bottle.
Flow Cell Compartment
The flow cell stage includes the flow cell latch, which secures the flow cell when it is closed. As the latch
closes, pins near the base of the latch align the flow cell ports with fluidics connections.
Document # 1000000002695 v02 Material # 20014309
For Research Use Only. Not for use in diagnostic procedures.
2
MiniSeq System Guide

illumina MiniSeq Specifications

General

Instrument Type	Benchtop Sequencer
Sequencing Technology	Sequencing by Synthesis (SBS)
Read Length	Up to 2 x 150 bp
Output	Up to 7.5 Gb
Run Time	4 - 24 hours
Data Quality	≥ 80% bases ≥ Q30
Maximum Reads	25 million
Sample Capacity	1
Applications	Targeted Gene Sequencing, Small Genome Sequencing, Amplicon Sequencing, Gene Expression Profiling
Power Requirements	100-240 V, 50-60 Hz
Operating Temperature	19°C to 25°C
Operating Humidity	Non-condensing, 20% to 80% RH