illumina MiniSeq User Manual

Chapter 1 Overview
Introduction 1
Additional Resources 1
Instrument Components 2
Sequencing Consumables Overview 5
Preinstalled Databases and Genomes 7
Introduction
The Illumina
®
MiniSeq™ System provides high-quality, industry-standard Illumina sequencing technology with
the convenience of an easy-to-use cost-effective desktop system.
Features
u High-quality sequencing—The MiniSeq System enables small genome, amplicon, targeted enrichment,
and RNA sequencing using low library volumes.
u MiniSeq system software—The MiniSeq System includes a suite of integrated software that controls
instrument operations, processes images, and generates base calls. The suite includes on-instrument
data analysis software and data transfer tools for analysis using other options, such as BaseSpace.
u On-instrument data analysis—The Local Run Manager software analyzes run data according to the
analysis module specified for the run. A suite of analysis modules are included in the software.
u BaseSpace
®
integration—The sequencing workflow is integrated with BaseSpace, the Illumina
genomics computing environment for run monitoring, data analysis, storage, and collaboration.
Output files are streamed in real time to BaseSpace or BaseSpace Onsite for analysis.
u Convenient consumable loading—A clamping mechanism positions the flow cell as it is loaded onto the
instrument. A single-use prefilled reagent cartridge provides reagents required for a run and subsequent
instrument wash. The flow cell and reagent cartridge include integrated identification to enable accurate
tracking.
Additional Resources
The MiniSeq System support pages on the Illumina website provide additional resources. These resources
include software, training, compatible products, and the following documentation. Always check support
pages for the latest versions.
Resource Description
Custom Protocol Selector A wizard for generating customized end-to-end documentation that is tailored to the
library prep method, run parameters, and analysis method used for the sequencing run.
MiniSeq System Site Prep
Guide (document #
1000000002696)
Provides specifications for laboratory space, electrical requirements, and environmental
considerations.
MiniSeq System Safety and
Compliance Guide
(document #
1000000002698)
Provides information about operational safety considerations, compliance statements,
and instrument labeling.
RFID Reader Compliance
Guide (document #
1000000002699)
Provides information about the RFID reader in the instrument, compliance certifications,
and safety considerations.
Document # 1000000002695 v02 Material # 20014309
For Research Use Only. Not for use in diagnostic procedures.
1

illumina MiniSeq Specifications

General

Instrument Type	Benchtop Sequencer
Sequencing Technology	Sequencing by Synthesis (SBS)
Read Length	Up to 2 x 150 bp
Output	Up to 7.5 Gb
Run Time	4 - 24 hours
Data Quality	≥ 80% bases ≥ Q30
Maximum Reads	25 million
Sample Capacity	1
Applications	Targeted Gene Sequencing, Small Genome Sequencing, Amplicon Sequencing, Gene Expression Profiling
Power Requirements	100-240 V, 50-60 Hz
Operating Temperature	19°C to 25°C
Operating Humidity	Non-condensing, 20% to 80% RH