illumina MiniSeq User Manual

Appendix A Troubleshooting
Troubleshooting Files 38
Automatic Check Errors 39
RTA Errors 40
Rehybridization Workflow 41
System Check 42
Network Configuration Settings 45
Custom Genomes 46
Shut Down the Instrument 46
Troubleshooting Files
Key File Folder Description
Run information file
(RunInfo.xml)
Root folder Contains the following information:
• Run name
• Number of cycles in the run
• Number of cycles in each read
• Whether the read is an indexed read
• Number of swaths and tiles on the flow cell
Run parameters file
(RunParameters.xml)
Root folder Contains information about run parameters and run components.
Information includes the RFID, serial number, lot number, and expiration
date.
RTA configuration file
(RTAConfiguration.xml)
Data\Intensities Contains the RTA configuration settings for the run.
The RTAConfiguration.xml file is created at the beginning of the run.
InterOp files (*.bin) InterOp Binary reporting files used for Sequencing Analysis Viewer.
InterOp files are updated throughout the run.
Log files Logs Log files describe each step performed by the instrument for each cycle,
and list software and firmware versions used with the run. The file named
[InstrumentName]_CurrentHardware.csv lists the serial numbers of
instrument components.
Error log files
(*ErrorLog*.txt)
RTALogs Log of RTA errors.
Error log files are updated whenever an error occurs.
Global log files
(*GlobalLog*.tsv)
RTALogs Log of all RTA events.
Global log files are updated throughout the run.
Lane log files
(*LaneLog*.txt)
RTALogs Log RTA processing events.
Lane log files are updated throughout the run.
Troubleshooting Resources
For technical questions, visit the MiniSeq System support pages on the Illumina website. The support pages
provide access to documentation, downloads, and frequently asked questions.
Log in to your MyIllumina account for access to support bulletins.
For run quality or performance problems, contact Illumina Technical Support. See Technical Assistance on
page 57.
Consider sharing a link to the run summary in BaseSpace with Illumina Technical Support to facilitate
troubleshooting.
Document # 1000000002695 v02 Material # 20014309
For Research Use Only. Not for use in diagnostic procedures.
38

illumina MiniSeq Specifications

General

Instrument Type	Benchtop Sequencer
Sequencing Technology	Sequencing by Synthesis (SBS)
Read Length	Up to 2 x 150 bp
Output	Up to 7.5 Gb
Run Time	4 - 24 hours
Data Quality	≥ 80% bases ≥ Q30
Maximum Reads	25 million
Sample Capacity	1
Applications	Targeted Gene Sequencing, Small Genome Sequencing, Amplicon Sequencing, Gene Expression Profiling
Power Requirements	100-240 V, 50-60 Hz
Operating Temperature	19°C to 25°C
Operating Humidity	Non-condensing, 20% to 80% RH