illumina MiniSeq User Manual

Position Description
13, 14, and 15 Reserved for optional custom primers
16 Load libraries
Removable Reservoir in Position #9
The prefilled reagent cartridge includes a denaturation reagent in position #9 that contains formamide. To
facilitate safe disposal of any unused reagent after the sequencing run, this reservoir is removable. For more
information, see Remove Used Reservoir from Position #9 on page 30.
Preinstalled Databases and Genomes
For most analysis methods, a reference is required to perform alignment. Several reference databases and
genomes are preinstalled on the instrument computer.
Preinstalled Description
Databases
• miRbase for human
• dbSNP for human
• RefGene for human
Genomes
• Arabidopsis thaliana
• cow ( Bos taurus )
• E. coli strain DH10B
• E. coli strain MG1655
• fruit fly ( Drosophila melanogaster )
• human ( Homo sapiens ) build hg19
• mouse ( Mus musculus )
• PhiX
• rat ( Rattus norvegicus )
• Rhodobacter sphaeroides 2.4.1
• yeast ( Saccharomyces cerevisiae S288C)
• Staphylococcus aureus NCTC 8325
Document # 1000000002695 v02 Material # 20014309
For Research Use Only. Not for use in diagnostic procedures.
7
MiniSeq System Guide

illumina MiniSeq Specifications

General

Instrument Type	Benchtop Sequencer
Sequencing Technology	Sequencing by Synthesis (SBS)
Read Length	Up to 2 x 150 bp
Output	Up to 7.5 Gb
Run Time	4 - 24 hours
Data Quality	≥ 80% bases ≥ Q30
Maximum Reads	25 million
Sample Capacity	1
Applications	Targeted Gene Sequencing, Small Genome Sequencing, Amplicon Sequencing, Gene Expression Profiling
Power Requirements	100-240 V, 50-60 Hz
Operating Temperature	19°C to 25°C
Operating Humidity	Non-condensing, 20% to 80% RH