illumina MiniSeq User Manual

L001—Focus images
InstrumentAnalyticsLogs—Log files describing instrument analytics steps
InterOp—Binary files used by Sequencing Analysis Viewer
Logs—Log files describing operational steps
Recipe—Run-specific recipe file named with reagent cartridge ID
RTALogs—Log files describing analysis steps
RTAComplete.xml
RTAConfiguration.xml
RunInfo.xml
RunNotes.xml
RunParameters.xml
Analysis Input File Requirements
Local Run Manager requires the following files generated during the sequencing run to perform analysis or to
requeue analysis. Some analysis modules require additional input files to perform analysis. For more
information, see the workflow guide for the analysis module you are using.
File Name / Type Description
RTAComplete.txt A marker file that indicates RTA processing is complete. The presence of this file triggers
Local Run Manager to queue analysis.
RunInfo.xml Contains high-level run information, such as the number of reads and cycles in the
sequencing run, and whether a read is indexed.
Base call files (*.bcl) Contains the base call and encoded quality score for every cluster for each tile,
aggregated in a file for each cycle.
Filter files (*.filter) Specifies whether a cluster passed filters. Filter information is aggregated into a filter file
for each read.
Cluster location files (*.locs) Contains the XY coordinates for every cluster for each tile, aggregated into a cluster
location file
Document # 1000000002695 v02 Material # 20014309
For Research Use Only. Not for use in diagnostic procedures.
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MiniSeq System Guide

illumina MiniSeq Specifications

General

Instrument Type	Benchtop Sequencer
Sequencing Technology	Sequencing by Synthesis (SBS)
Read Length	Up to 2 x 150 bp
Output	Up to 7.5 Gb
Run Time	4 - 24 hours
Data Quality	≥ 80% bases ≥ Q30
Maximum Reads	25 million
Sample Capacity	1
Applications	Targeted Gene Sequencing, Small Genome Sequencing, Amplicon Sequencing, Gene Expression Profiling
Power Requirements	100-240 V, 50-60 Hz
Operating Temperature	19°C to 25°C
Operating Humidity	Non-condensing, 20% to 80% RH