illumina MiniSeq User Manual

Custom Genomes
You can upload your own reference in FASTA format to the instrument computer. You can upload several
single FASTA files or a single multi-FASTA file (recommended), but not a combination of both.
To troubleshoot issues with a custom genome file, confirm the following requirements.
1 Make sure that the file uses a *.fa or *.fasta extension and that it is stored in a dedicated folder for the
reference.
2 Make sure that the chromosome name does not contain any of following characters:
# - ? ( ) [ ] / \ = + < > : ; " ' , * ^ | &
For best results, use only alpha-numeric characters as chromosome names.
Shut Down the Instrument
Under normal conditions, there is no reason to shut down the instrument.
1 Select Manage Instrument.
2 Select Shutdown options.
3 Select Shut down.
The command safely shuts down the software and turns off instrument power. Wait at least 60 seconds
before turning on the instrument again. A wash is required before the next sequencing run.
CAUTION
Do not relocate the instrument. Moving the instrument improperly can affect the optical alignment and
compromise data integrity. If you have to relocate the instrument, contact your Illumina representative.
Document # 1000000002695 v02 Material # 20014309
For Research Use Only. Not for use in diagnostic procedures.
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MiniSeq System Guide

illumina MiniSeq Specifications

General

Instrument Type	Benchtop Sequencer
Sequencing Technology	Sequencing by Synthesis (SBS)
Read Length	Up to 2 x 150 bp
Output	Up to 7.5 Gb
Run Time	4 - 24 hours
Data Quality	≥ 80% bases ≥ Q30
Maximum Reads	25 million
Sample Capacity	1
Applications	Targeted Gene Sequencing, Small Genome Sequencing, Amplicon Sequencing, Gene Expression Profiling
Power Requirements	100-240 V, 50-60 Hz
Operating Temperature	19°C to 25°C
Operating Humidity	Non-condensing, 20% to 80% RH