illumina MiniSeq User Manual

Chapter 4 Maintenance
Introduction 32
Perform a Manual Instrument Wash 32
Software Updates 35
Introduction
Maintenance procedures include manual instrument washes and system software updates when available.
No other periodic maintenance is required.
u Instrument washes—An automatic post-run wash after each sequencing run maintains instrument
performance. However, a manual instrument wash is required under certain conditions. See Perform a
Manual Instrument Wash on page 32.
u Software updates—When a new version of the system software suite is available, you can update the
software automatically through a connection to BaseSpace or manually after downloading the installer
from the Illumina website. See Software Updates on page 35.
Preventive Maintenance
Illumina recommends that you schedule a preventive maintenance service each year. If you are not under a
service contract, contact your Territory Account Manager or Illumina Technical Support to arrange for a
billable preventive maintenance service.
Perform a Manual Instrument Wash
Manual instrument wash options include the Quick Wash and the Manual Post-Run Wash.
Wash Types Description
Quick Wash
Duration: 20 minutes
A quick wash is required every 7 days that the instrument is idle or after an instrument
shutdown.
The wash flushes the system with a user-supplied wash solution of laboratory-grade
water and Tween 20.
Manual Post-Run Wash
Duration: 90 minutes
A manual post-run wash is required when the automatic post-run wash is not performed,
such as when a run is ended early and the flow cell is saved for later rehybridization.
The wash flushes the system with user-supplied 0.12% sodium hypochlorite and a wash
solution of laboratory-grade water and Tween 20.
NOTE
Always use a fresh dilution of NaOCl prepared within the last 24hours. If you make a volume larger than 1ml,
store the remaining dilution at 2°C to 8°C for use within the next 24 hours. Otherwise, discard the remaining
dilution of NaOCl.
A manual instrument wash requires the wash cartridge and a wash flow cell provided with the instrument.
Alternatively, you can use a used flow cell for the wash.
Document # 1000000002695 v02 Material # 20014309
For Research Use Only. Not for use in diagnostic procedures.
32

illumina MiniSeq Specifications

General

Instrument Type	Benchtop Sequencer
Sequencing Technology	Sequencing by Synthesis (SBS)
Read Length	Up to 2 x 150 bp
Output	Up to 7.5 Gb
Run Time	4 - 24 hours
Data Quality	≥ 80% bases ≥ Q30
Maximum Reads	25 million
Sample Capacity	1
Applications	Targeted Gene Sequencing, Small Genome Sequencing, Amplicon Sequencing, Gene Expression Profiling
Power Requirements	100-240 V, 50-60 Hz
Operating Temperature	19°C to 25°C
Operating Humidity	Non-condensing, 20% to 80% RH