illumina MiniSeq User Manual

Figure 26 Wash Cartridge
Prepare for a Manual Post-Run Wash
1 Combine the following volumes to result in 0.12% NaOCl.
u 5% NaOCl (31µl)
u Laboratory-grade water (1269 µl)
2 Add 1.3 ml of 0.12% NaOCl to the wash cartridge.
The correct reservoir is equivalent to position #31 on the prefilled reagent cartridge.
Figure 27 Positions for NaOCl and Wash Solution
A 0.12% NaOCl
B Wash solution
3 Combine the following volumes to result in a 0.05% Tween 20 wash solution.
u 100% Tween 20 (40 µl)
u Laboratory-grade water (80 ml)
4 Add 80 ml wash solution to the wash cartridge.
The correct reservoir is equivalent to position #40 on the prefilled reagent cartridge.
5 From the Home screen, select Perform Wash, and then select Manual post-run wash.
Document # 1000000002695 v02 Material # 20014309
For Research Use Only. Not for use in diagnostic procedures.
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MiniSeq System Guide

illumina MiniSeq Specifications

General

Instrument Type	Benchtop Sequencer
Sequencing Technology	Sequencing by Synthesis (SBS)
Read Length	Up to 2 x 150 bp
Output	Up to 7.5 Gb
Run Time	4 - 24 hours
Data Quality	≥ 80% bases ≥ Q30
Maximum Reads	25 million
Sample Capacity	1
Applications	Targeted Gene Sequencing, Small Genome Sequencing, Amplicon Sequencing, Gene Expression Profiling
Power Requirements	100-240 V, 50-60 Hz
Operating Temperature	19°C to 25°C
Operating Humidity	Non-condensing, 20% to 80% RH