Prepare for a Quick Wash
1 Combine the following volumes to result in a 0.05% Tween 20 wash solution.
u 100% Tween 20 (20 µl)
u Laboratory-grade water (40 ml)
2 Add 40 ml wash solution to the wash cartridge.
The correct reservoir is equivalent to position #40 on the prefilled reagent cartridge.
Figure 28 Position for Wash Solution
A Wash solution
3 From the Home screen, select Perform wash, and then select Quick Wash.
Load the Wash Flow Cell and Wash Cartridge
1 Load the wash flow cell. Close the flow cell clamp and flow cell door.
NOTE
Alternatively, you can load a used flow cell.
2 Remove the used reagent cartridge from the previous run, if present.
3 Load the prepared wash cartridge.
4 Remove the spent reagents bottle and discard the contents in accordance with applicable standards.
WARNING
This set of reagents contains potentially hazardous chemicals. Personal injury can occur through
inhalation, ingestion, skin contact, and eye contact. Wear protective equipment, including eye
protection, gloves, and laboratory coat appropriate for risk of exposure. Handle used reagents as
chemical waste and discard in accordance with applicable regional, national, and local laws and
regulations. For additional environmental, health, and safety information, see the SDS at
support.illumina.com/sds.html.
5 Slide the empty spent reagents bottle into the compartment until it stops.
6 Close the reagent compartment door.
Document # 1000000002695 v02 Material # 20014309
For Research Use Only. Not for use in diagnostic procedures.
34
MiniSeq System Guide
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