illumina MiniSeq User Manual

Prepare Consumables
Prepare the Reagent Cartridge
1 Remove thereagent cartridge from -25°C to -15°C storage.
2 Thaw reagents using the following water bath options. Do not submerge the cartridge. When thawed, dry
the base before proceeding.
Method Time to Thaw Stability Limit
37°C water bath 35 minutes Up to 2 hours
Room temperature water bath
(19°C to 25°C)
90 minutes Up to 24 hours
If multiple cartridges are thawing in the same water bath, allow additional thawing time.
Alternatively, thaw reagents using the following options.
Method Time to Thaw Stability Limit
Room temperature air
(19°C to 25°C)
5 hours Up to 24 hours
Chilled at 2°C to 8°C 18 hours Up to 72 hours
3 Invert the cartridge 5 times to mix reagents.
4 Inspect the large reservoirs from the bottom of the cartridge to make sure that reagents have thawed
and the reservoirs are free of ice crystals.
5 Gently tap on the bench to reduce air bubbles.
Prepare the Flow Cell
1 Remove a new flow cell package from 2°C to 8°C storage.
2 Set the unopened flow cell package aside at room temperature for 30 minutes.
NOTE
Avoid repeated cooling and warming of the flow cell.
3 Remove the flow cell container from the foil package.
4 Put on a new pair of powder-free gloves.
5 Grip the flow cell by the plastic cartridge and remove it from the container.
Figure 9 Remove Flow Cell
6 Clean the glass surface of the flow cell with a lint-free alcohol wipe.
Document # 1000000002695 v02 Material # 20014309
For Research Use Only. Not for use in diagnostic procedures.
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MiniSeq System Guide

illumina MiniSeq Specifications

General

Instrument Type	Benchtop Sequencer
Sequencing Technology	Sequencing by Synthesis (SBS)
Read Length	Up to 2 x 150 bp
Output	Up to 7.5 Gb
Run Time	4 - 24 hours
Data Quality	≥ 80% bases ≥ Q30
Maximum Reads	25 million
Sample Capacity	1
Applications	Targeted Gene Sequencing, Small Genome Sequencing, Amplicon Sequencing, Gene Expression Profiling
Power Requirements	100-240 V, 50-60 Hz
Operating Temperature	19°C to 25°C
Operating Humidity	Non-condensing, 20% to 80% RH