illumina MiniSeq User Manual

NOTE
Do not open the reagent compartment door or the flow cell compartment door during the automated
check or during the sequencing run.
Review Automated Check
1 Review the automated check results.
u To stop a check in progress, select Cancel.
u For any items that do not pass, an action is required before you can proceed. See Automatic Check
Errors on page 39.
u To restart the check, select Retry. The check resumes at the first incomplete or failed check.
2 To start the run, select from the following options.
u If the system is not configured to start automatically after a successful check, select Start.
u If the system is configured to start automatically after a successful check, the sequencing run begins
automatically. You do not have to be present. However, if any errors occur during the check, the run
does not begin automatically.
Monitor Run Progress
1 Monitor run progress, intensities, and quality scores as metrics appear on the screen.
Figure 23 Sequencing Run Progress and Metrics
A Run progress—Shows the current step and number of cycles completed for each read. The progress bar is
not proportional to the run rate of each step.
B Q-Score—Shows the distribution of quality scores (Q-scores). See Quality Scoring on page 51.
C Intensity—Shows the value of cluster intensities of the 90
th
percentile for each tile. Plot colors indicate each
base: red is A, green is C, blue is G, and black is T.
D Cluster density (K/mm²)—Shows the number of clusters detected for the run.
E Clusters passing filter (%)—Shows the percentage of clusters passing filter. See Clusters Passing Filter on
page 50.
F Estimated yield (Gb)—Shows the number of bases projected for the run.
G Data transfer status—Shows the status of data transfer based on analysis configuration.
H Time to completion—Shows the run completion date and time (yyyy-mm-dd hh:mm).
Document # 1000000002695 v02 Material # 20014309
For Research Use Only. Not for use in diagnostic procedures.
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MiniSeq System Guide

illumina MiniSeq Specifications

General

Instrument Type	Benchtop Sequencer
Sequencing Technology	Sequencing by Synthesis (SBS)
Read Length	Up to 2 x 150 bp
Output	Up to 7.5 Gb
Run Time	4 - 24 hours
Data Quality	≥ 80% bases ≥ Q30
Maximum Reads	25 million
Sample Capacity	1
Applications	Targeted Gene Sequencing, Small Genome Sequencing, Amplicon Sequencing, Gene Expression Profiling
Power Requirements	100-240 V, 50-60 Hz
Operating Temperature	19°C to 25°C
Operating Humidity	Non-condensing, 20% to 80% RH