illumina MiniSeq User Manual

Reagent Cartridge Overview
The reagent cartridge is a single-use consumable with foil-sealed reservoirs that are prefilled with clustering,
sequencing, and wash reagents.
Figure 5 Reagent Cartridge
The reagent cartridge includes a designated reservoir for loading prepared libraries. After the run begins,
libraries are transferred automatically from the reagent cartridge to the flow cell.
WARNING
This set of reagents contains potentially hazardous chemicals. Personal injury can occur through inhalation,
ingestion, skin contact, and eye contact. Wear protective equipment, including eye protection, gloves, and
laboratory coat appropriate for risk of exposure. Handle used reagents as chemical waste and discard in
accordance with applicable regional, national, and local laws and regulations. For additional environmental,
health, and safety information, see the SDS at support.illumina.com/sds.html.
Store the reagent cartridge at -25°C to -15°C until use. For more information, see Prepare the Reagent
Cartridge on page 19.
Reserved Reservoirs
Figure 6 Numbered Reservoirs
Document # 1000000002695 v02 Material # 20014309
For Research Use Only. Not for use in diagnostic procedures.
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MiniSeq System Guide

illumina MiniSeq Specifications

General

Instrument Type	Benchtop Sequencer
Sequencing Technology	Sequencing by Synthesis (SBS)
Read Length	Up to 2 x 150 bp
Output	Up to 7.5 Gb
Run Time	4 - 24 hours
Data Quality	≥ 80% bases ≥ Q30
Maximum Reads	25 million
Sample Capacity	1
Applications	Targeted Gene Sequencing, Small Genome Sequencing, Amplicon Sequencing, Gene Expression Profiling
Power Requirements	100-240 V, 50-60 Hz
Operating Temperature	19°C to 25°C
Operating Humidity	Non-condensing, 20% to 80% RH