illumina MiniSeq User Manual

Figure 16 Seal the Spent Reagents Bottle
3 Discard the contents in accordance with applicable standards.
WARNING
This set of reagents contains potentially hazardous chemicals. Personal injury can occur through
inhalation, ingestion, skin contact, and eye contact. Wear protective equipment, including eye
protection, gloves, and laboratory coat appropriate for risk of exposure. Handle used reagents as
chemical waste and discard in accordance with applicable regional, national, and local laws and
regulations. For additional environmental, health, and safety information, see the SDS at
support.illumina.com/sds.html.
4 With the threaded cap removed, slide the empty spent reagents bottle into the compartment until it
stops.
5 Close the compartment door and select Next.
Enter Run Parameters
1 Enter a run name of your preference.
2 [Optional] Enter a library ID of your preference.
3 From the Recipe drop-down list, select a recipe. Only compatible recipes are listed.
4 Select a read type, either Single read or Paired end.
5 Enter the number of cycles for each read in the sequencing run.
u Read 1—Enter a value up to 151 cycles.
u Index 1—Enter the number of cycles required for the Index 1 (i7) primer.
u Index 2—Enter the number of cycles required for the Index 2 (i5) primer.
u Read 2—Enter a value up to 151 cycles. This value is typically the same number of cycles as Read 1.
The control software confirms the number of specified cycles using the following criteria:
u Total cycles do not exceed the maximum cycles allowed based on the reagent cartridge loaded for
the run.
u Cycles for Read 1 are greater than the 5 cycles required for template generation.
u Index Read cycles do not exceed Read 1 and Read 2 cycles.
NOTE
Make sure that you specify the appropriate number of Index Read cycles for the libraries that you are
sequencing. For more information, see the library prep documentation.
Document # 1000000002695 v02 Material # 20014309
For Research Use Only. Not for use in diagnostic procedures.
23
MiniSeq System Guide

illumina MiniSeq Specifications

General

Instrument Type	Benchtop Sequencer
Sequencing Technology	Sequencing by Synthesis (SBS)
Read Length	Up to 2 x 150 bp
Output	Up to 7.5 Gb
Run Time	4 - 24 hours
Data Quality	≥ 80% bases ≥ Q30
Maximum Reads	25 million
Sample Capacity	1
Applications	Targeted Gene Sequencing, Small Genome Sequencing, Amplicon Sequencing, Gene Expression Profiling
Power Requirements	100-240 V, 50-60 Hz
Operating Temperature	19°C to 25°C
Operating Humidity	Non-condensing, 20% to 80% RH